TY - JOUR
T1 - Magnetic properties and cytocompatibility of transition-metal-incorporated hydroxyapatite
AU - Zilm, Michael E.
AU - Yu, Le
AU - Hines, William A.
AU - Wei, Mei
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - A detailed magnetization study, along with an assessment of the cellular proliferation, has been carried out on transition-metal-doped hydroxyapatite (HA), Ca10−xMx(PO4)6(OH)2, where M = Mn, Co, and Fe. In particular, a series of MnHA powder samples with an x value of 0.04 ≤ x ≤ 1.21, one CoHA (x = 0.48) and one FeHA sample (x = 1.06) were synthesized using a wet chemical method along with an ion-exchange procedure. Characterization by transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDXS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) indicated that the substitution of M elements does not change the morphology and crystalline structure of pure HA that showing a single phased HA nano-rod. In every case, the magnetization isotherms for 10 K ≤ T ≤ 300 K were linear through the origin characteristic of a paramagnetic response with no indication of superparamagnetic behavior, hysteresis, or magnetic ordering. The magnetic behavior for all samples could be fit to the Curie-Weiss law yielding values for the M ion magnetic moments. The Mn2+ magnetic moments were close to the spin-only value of S = 5/2 or 5.92 μB, while the Co2+ moment (4.41 μB) was larger than the spin-only value for S = 3/2, indicating an orbital contribution due to incomplete quenching. The magnetic behavior for the FeHA sample showed a possible spin-state transition. In addition, no statistically significant differences were observed when cells were treated with the same dose of HA or MnHA up to 50 μg/mL, suggesting that the substituted Mn introduces no cytotoxicity to the HA powders.
AB - A detailed magnetization study, along with an assessment of the cellular proliferation, has been carried out on transition-metal-doped hydroxyapatite (HA), Ca10−xMx(PO4)6(OH)2, where M = Mn, Co, and Fe. In particular, a series of MnHA powder samples with an x value of 0.04 ≤ x ≤ 1.21, one CoHA (x = 0.48) and one FeHA sample (x = 1.06) were synthesized using a wet chemical method along with an ion-exchange procedure. Characterization by transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDXS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) indicated that the substitution of M elements does not change the morphology and crystalline structure of pure HA that showing a single phased HA nano-rod. In every case, the magnetization isotherms for 10 K ≤ T ≤ 300 K were linear through the origin characteristic of a paramagnetic response with no indication of superparamagnetic behavior, hysteresis, or magnetic ordering. The magnetic behavior for all samples could be fit to the Curie-Weiss law yielding values for the M ion magnetic moments. The Mn2+ magnetic moments were close to the spin-only value of S = 5/2 or 5.92 μB, while the Co2+ moment (4.41 μB) was larger than the spin-only value for S = 3/2, indicating an orbital contribution due to incomplete quenching. The magnetic behavior for the FeHA sample showed a possible spin-state transition. In addition, no statistically significant differences were observed when cells were treated with the same dose of HA or MnHA up to 50 μg/mL, suggesting that the substituted Mn introduces no cytotoxicity to the HA powders.
UR - http://www.scopus.com/inward/record.url?scp=85042744810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042744810&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2018.02.018
DO - 10.1016/j.msec.2018.02.018
M3 - Article
C2 - 29549940
AN - SCOPUS:85042744810
SN - 0928-4931
VL - 87
SP - 112
EP - 119
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -