Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature

Eduard C. Nedea, Dmitriy Markov, Tatyana Naryshkina, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We localized five rpoC (β') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed β' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpbl-1. Thus, distinct, predominantly conserved β' residues participate in interactions holding RNA polymerase together.

Original languageEnglish (US)
Pages (from-to)2663-2665
Number of pages3
JournalJournal of bacteriology
Volume181
Issue number8
DOIs
StatePublished - Apr 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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