Lipase bound cellulose nanofibrous membrane via Cibacron Blue F3GA affinity ligand

Ping Lu, You Lo Hsieh

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Cellulose (Cell) nanofibrous membranes were prepared by nucleophilic reaction of the cellulose hydroxyl with the triazinyl chloride of Cibacron Blue F3GA (CB) ligand and studied as affinity membranes for lipase enzyme. Cell nanofibrous membranes containing fibers with 200 nm average diameters were prepared by electrospinning of cellulose acetate (CA), followed by alkaline hydrolysis. The CB capacity of the Cell nanofibrous membranes was optimized by lengthening the nucleophilic reaction time and increasing CB concentration and ionic strength. The equilibrium adsorption isotherms of CB on the Cell nanofibrous membranes followed a typical Langmuir monolayer adsorption behavior. At 242 mg CB/g of Cell, the maximum lipase adsorption capacity (qm) and the dissociation constant (Kd) values were 41.02 mg/g and 0.25 mg/mL, respectively. Optimal lipase adsorption capacity was obtained at pH 4.0, its isoelectric point, with added NaCl on Cell membranes 86 mg CB capacity per g of Cell. A facile lipase loading capacity of 16.21 mg/g of CB-Cell was achieved under moderated conditions and could be optimized to reach at least 150 mg/g. The CB-Cell bound lipase had similar catalytic rate and retained 86.2% activity as in its free form. These findings clearly show that the CB bound Cell nanofibrous membrane is a highly efficient ultra-high specific porous support for lipase enzyme and is potentially versatile for immobilizing other enzymes and as affinity membrane for proteins.

Original languageEnglish (US)
Pages (from-to)288-296
Number of pages9
JournalJournal of Membrane Science
Volume330
Issue number1-2
DOIs
StatePublished - Mar 2 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Materials Science(all)
  • Physical and Theoretical Chemistry
  • Filtration and Separation

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