Isolation of growth suppressors from a cDNA expression library

Dimitri G. Pestov, Tatiana M. Grzeszkiewicz, Lester F. Lau

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We describe an experimental procedure for the isolation of growth inhibitory sequences from a complex cDNA library. This approach first takes advantage of the SETGAP technique (selectable expression of transient growth arrest phenotype) to enrich for growth inhibitory sequences, followed by a screening procedure to identify individual cDNAs that inhibit cell proliferation. Here we provide a detailed description of the experimental protocol and report the characterization of two cDNA sequences isolated in our initial screen of a mouse cDNA library. One of these cDNAs encodes the mouse ubiquitin-conjugation enzyme UbcM2. The other encodes a truncated form of a novel WD40 repeat protein, named Bop1, which is conserved from yeast to human. Together, these results demonstrate a new approach for the isolation of growth suppressors from cDNA libraries, and identify a previously unknown gene likely to be involved in growth control.

Original languageEnglish (US)
Pages (from-to)3187-3197
Number of pages11
JournalOncogene
Volume17
Issue number24
DOIs
StatePublished - Dec 17 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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