TY - JOUR
T1 - Investigating the correlation between DNA methylation and immune‑associated genes of lung adenocarcinoma based on a competing endogenous RNA network
AU - Chang, Chun
AU - Kong, Wei
AU - Mou, Xiaoyang
AU - Wang, Shuaiqun
N1 - Funding Information:
The present study was supported by the national natural Science Foundation of china (grant no. 61803257) and natural Science Foundation of Shanghai (grant no. 18Zr1417200).
Funding Information:
The authors would like to thank dr deng Jin and Mr Wei Boyang (both Shanghai Maritime university, Shanghai, china) for their technical assistance and writing assistance during the research process. The present study was supported by the national natural Science Foundation of china (grant no. 61803257) and natural Science Foundation of Shanghai (grant no. 18Zr1417200).
Publisher Copyright:
© 2020 Spandidos Publications. All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - inrecentyears,therehavebeenmajorbreakthroughs in immunotherapies for the treatment of cancer. However, different patients have different responses to immunotherapy. numerous studies have shown that the accumulation of epigenetic abnormalities, such as dna methylation, serve an important role in the immune response of lung adenocarcinoma (luad). To investigate the effects of dna methylation on tumor immunity with survival and prognosis, relevant studies can be performed based on the regulatory mechanisms of rna molecules. For example, long non-coding rnas (lncrnas), which regulate gene expression through epigenetic levels. By constructing an immune-associated competitive endogenous rna (cerna) network, the present study identified the regulatory associations among 3 key immune-associations mrnas, 2 micrornas (mirs) and 29 lncrnas that were closely associated with the prognosis of patients with luad. The molecular biology analysis indicated that hypomethylation of the 1101320-1104290 regions of chromosome 1 resulted in the low expression levels of LINC00337 and that LINC00337 may affect the expression levels of CHEK1 by competitively binding with human (has)‑miR‑373 and hsa‑miR‑195. Therefore, abnormal dna methylation in lncrna-associated regions caused their abnormal expression levels, which further affected the interactions between rna molecules. The interactions between these rna molecules may have regulatory effects on tumor immunity and the prognosis of patients with luad.
AB - inrecentyears,therehavebeenmajorbreakthroughs in immunotherapies for the treatment of cancer. However, different patients have different responses to immunotherapy. numerous studies have shown that the accumulation of epigenetic abnormalities, such as dna methylation, serve an important role in the immune response of lung adenocarcinoma (luad). To investigate the effects of dna methylation on tumor immunity with survival and prognosis, relevant studies can be performed based on the regulatory mechanisms of rna molecules. For example, long non-coding rnas (lncrnas), which regulate gene expression through epigenetic levels. By constructing an immune-associated competitive endogenous rna (cerna) network, the present study identified the regulatory associations among 3 key immune-associations mrnas, 2 micrornas (mirs) and 29 lncrnas that were closely associated with the prognosis of patients with luad. The molecular biology analysis indicated that hypomethylation of the 1101320-1104290 regions of chromosome 1 resulted in the low expression levels of LINC00337 and that LINC00337 may affect the expression levels of CHEK1 by competitively binding with human (has)‑miR‑373 and hsa‑miR‑195. Therefore, abnormal dna methylation in lncrna-associated regions caused their abnormal expression levels, which further affected the interactions between rna molecules. The interactions between these rna molecules may have regulatory effects on tumor immunity and the prognosis of patients with luad.
UR - http://www.scopus.com/inward/record.url?scp=85090174087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85090174087&partnerID=8YFLogxK
U2 - 10.3892/mmr.2020.11445
DO - 10.3892/mmr.2020.11445
M3 - Article
C2 - 32945447
AN - SCOPUS:85090174087
VL - 22
SP - 3173
EP - 3182
JO - Molecular Medicine Reports
JF - Molecular Medicine Reports
SN - 1791-2997
IS - 4
ER -