TY - JOUR
T1 - Increased numbers of CD23+CD21hi Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes
AU - Kuzin, Igor I.
AU - Kates, Stephen L.
AU - Ju, Yawen
AU - Zhang, Longze
AU - Rahimi, Homaira
AU - Wojciechowski, Wojciech
AU - Bernstein, Steven H.
AU - Burack, Richard
AU - Schwarz, Edward M.
AU - Bottaro, Andrea
N1 - Funding Information:
We are grateful to URMC Flow Cytometry SRL staff for technical support. The authors would also like to thank Allison McIntyre and Amy Battisti for assistance in collecting the human tissue, and Sarah Mack for technical assistance with the histology and immunohistochemistry. This work was supported by research grants from the National Institutes of Health, PHS awards K12 HD068373; R01 AR056702; P01 AI078907; and P30 AR061307.
Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2016/7/1
Y1 - 2016/7/1
N2 - A unique population of CD23+ CD21high B cells in inflamed nodes (Bin) has been shown to accumulate in lymph nodes (LNs) draining inflamed joints of TNF-transgenic (TNF-tg) mice. Bin cells contribute to arthritis flare in mice by distorting node architecture and hampering lymphatic flow, but their existence in human inflamed LNs has not yet been described. Here, we report the characterization of resident B-cell populations in fresh popliteal lymph nodes (PLNs) from patients with severe lower limb diseases (non-RA) and rheumatoid arthritis (RA) patients, and from banked, cryopreserved reactive and normal human LN single cell suspension samples. Bin-like B cells were shown to be significantly increased in reactive LNs, and strikingly elevated (>30% of total) in RA samples. Histopathology and immunofluorescence analyses were consistent with B follicular hyperplasia and histological alterations in RA vs. non-RA PLNs. This is the first description of Bin-like B cells in human inflamed LNs. Consistent with published mouse data, this population appears to be associated with inflammatory arthritis and distortion of LN architecture. Further analyses are necessary to assess the role of CD23+CD21hi Bin-like B cells in RA pathogenesis and arthritic flare.
AB - A unique population of CD23+ CD21high B cells in inflamed nodes (Bin) has been shown to accumulate in lymph nodes (LNs) draining inflamed joints of TNF-transgenic (TNF-tg) mice. Bin cells contribute to arthritis flare in mice by distorting node architecture and hampering lymphatic flow, but their existence in human inflamed LNs has not yet been described. Here, we report the characterization of resident B-cell populations in fresh popliteal lymph nodes (PLNs) from patients with severe lower limb diseases (non-RA) and rheumatoid arthritis (RA) patients, and from banked, cryopreserved reactive and normal human LN single cell suspension samples. Bin-like B cells were shown to be significantly increased in reactive LNs, and strikingly elevated (>30% of total) in RA samples. Histopathology and immunofluorescence analyses were consistent with B follicular hyperplasia and histological alterations in RA vs. non-RA PLNs. This is the first description of Bin-like B cells in human inflamed LNs. Consistent with published mouse data, this population appears to be associated with inflammatory arthritis and distortion of LN architecture. Further analyses are necessary to assess the role of CD23+CD21hi Bin-like B cells in RA pathogenesis and arthritic flare.
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U2 - 10.1002/eji.201546266
DO - 10.1002/eji.201546266
M3 - Article
C2 - 27105894
AN - SCOPUS:84978221228
VL - 46
SP - 1752
EP - 1757
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 7
ER -