TY - JOUR
T1 - Impact of processing parameters on the haemocompatibility of Bombyx mori silk films
AU - Seib, F. Philipp
AU - Maitz, Manfred F.
AU - Hu, Xiao
AU - Werner, Carsten
AU - Kaplan, David L.
N1 - Funding Information:
The authors would like to thank Dr. Ralf Zimmermann for electrokinetic analysis, Grit Ebert for technical assistance and Kate Sander for editing this manuscript. This work was supported by NIH grant P41 EB002520-05 (Tissue Engineering Resource Center) (DLK), FPS is supported by a Mildred Scheel Postdoctoral fellowship from the German Cancer Aid.
PY - 2012/2
Y1 - 2012/2
N2 - Silk has traditionally been used for surgical sutures due to its lasting strength and durability; however, the use of purified silk proteins as a scaffold material for vascular tissue engineering goes beyond traditional use and requires application-orientated biocompatibility testing. For this study, a library of Bombyx mori silk films was generated and exposed to various solvents and treatment conditions to reflect current silk processing techniques. The films, along with clinically relevant reference materials, were exposed to human whole blood to determine silk blood compatibility. All substrates showed an initial inflammatory response comparable to polylactide- co-glycolide (PLGA), and a low to moderate haemostasis response similar to polytetrafluoroethylene (PTFE) substrates. In particular, samples that were water annealed at 25 °C for 6 h demonstrated the best blood compatibility based on haemostasis parameters (e.g. platelet decay, thrombin-antithrombin complex, platelet factor 4, granulocytes-platelet conjugates) and inflammatory parameters (e.g. C3b, C5a, CD11b, surface-associated leukocytes). Multiple factors such as treatment temperature and solvent influenced the biological response, though no single physical parameter such as β-sheet content, isoelectric point or contact angle accurately predicted blood compatibility. These findings, when combined with prior in vivo data on silk, support a viable future for silk-based vascular grafts.
AB - Silk has traditionally been used for surgical sutures due to its lasting strength and durability; however, the use of purified silk proteins as a scaffold material for vascular tissue engineering goes beyond traditional use and requires application-orientated biocompatibility testing. For this study, a library of Bombyx mori silk films was generated and exposed to various solvents and treatment conditions to reflect current silk processing techniques. The films, along with clinically relevant reference materials, were exposed to human whole blood to determine silk blood compatibility. All substrates showed an initial inflammatory response comparable to polylactide- co-glycolide (PLGA), and a low to moderate haemostasis response similar to polytetrafluoroethylene (PTFE) substrates. In particular, samples that were water annealed at 25 °C for 6 h demonstrated the best blood compatibility based on haemostasis parameters (e.g. platelet decay, thrombin-antithrombin complex, platelet factor 4, granulocytes-platelet conjugates) and inflammatory parameters (e.g. C3b, C5a, CD11b, surface-associated leukocytes). Multiple factors such as treatment temperature and solvent influenced the biological response, though no single physical parameter such as β-sheet content, isoelectric point or contact angle accurately predicted blood compatibility. These findings, when combined with prior in vivo data on silk, support a viable future for silk-based vascular grafts.
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U2 - 10.1016/j.biomaterials.2011.10.063
DO - 10.1016/j.biomaterials.2011.10.063
M3 - Article
C2 - 22079005
AN - SCOPUS:81355147840
SN - 0142-9612
VL - 33
SP - 1017
EP - 1023
JO - Biomaterials
JF - Biomaterials
IS - 4
ER -