Imaging the bipolarity of myosin filaments with interferometric second Harmonic generation microscopy

Maxime Rivard, Charles André Couture, Amir K. Miri, Mathieu Laliberté, Antony Bertrand-Grenier, Luc Mongeau, François Légaré

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.

Original languageEnglish (US)
Pages (from-to)2078-2086
Number of pages9
JournalBiomedical Optics Express
Volume4
Issue number10
DOIs
StatePublished - 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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