Identification of three mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon

Glenn A. Doyle, X. Rebecca Sheng, Sharon S.J. Lin, Danielle M. Press, Dorothy E. Grice, Russell J. Buono, Thomas N. Ferraro, Wade H. Berrettini

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The mouse μ-opioid receptor gene, Oprm1, is recognized currently to contain 17 alternatively spliced exons that generate 24 splice variants encoding at least 11 morphine-binding isoforms of the receptor. Here, we identify three new MOR splice variants that contain a previously undescribed exon, exon 18, and provide evidence that they are expressed in two mouse strains. The transcripts containing the newly identified exon 18 encode two new putative μ-opioid receptor isoforms, MOR-1V and MOR-1W. In mouse Oprm1, exon 18 is located between the described exons 10 and 6. Exon 18 appears to be conserved in the rat genome between exons 4 and 7. A BLAST search of the non-redundant GenBank database suggests that human OPRM1 may also contain exon 18. Analysis of mouse brain mRNA by RT-PCR suggests that MOR-1Vii transcripts are expressed in all areas of the brain analyzed, whereas expression of MOR-1Vi transcripts was restricted to thalamus and striatum. MOR-1W transcripts are expressed most highly in the hypothalamus, thalamus and striatum. In summary, we have identified three brain expressed, alternatively spliced mouse MOR splice variants containing a novel exon and encoding new putative MOR isoforms, MOR-1V and MOR-1W.

Original languageEnglish (US)
Pages (from-to)135-147
Number of pages13
JournalGene
Volume388
Issue number1-2
DOIs
StatePublished - Feb 15 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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