Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis

Blanca Nieto, Sonia G. Gaspar, Giulia Moriggi, Dimitri G. Pestov, Xosé R. Bustelo, Mercedes Dosil

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit.

Original languageEnglish (US)
Article number156
JournalNature communications
Issue number1
StatePublished - Dec 1 2020

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General
  • General Physics and Astronomy


Dive into the research topics of 'Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis'. Together they form a unique fingerprint.

Cite this