Human milk fat globule membrane glycoproteins express blood group-related determinants primarily on mucin-like epithelial membrane antigens and gp70

A. S. Dion, Charlene Williams, M. Herlyn, P. Major

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Milk fat globule membranes (MFGM) were prepared from 21 human milks and dot-blotted. MFGM samples were compared with reference to 8 blood group-related antigens reactive with monoclonal antibodies and lectins. All preparations contained epithelial membrane antigen (EMA) and the majority was positive for type 1 Lewis a and b antigens, whereas only trace amounts of sialyl Lewis a were found. For type 2 Lewis antigens, most MFGM reacted intensely for X, but only weakly and infrequently for the Y antigen. Reactivities for H wee also infrequent and antigens related to A and/or B (types 1 and 2) were not found. Western blot analyses established that these antigenic determinants were borne mainly by mucin-like components and gp70.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalBiochemistry International
Volume22
Issue number2
StatePublished - Dec 1 1990

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Mucin-1
Membrane Glycoproteins
Mucins
Human Milk
Blood Group Antigens
Antigens
Membranes
Lectins
Epitopes
Western Blotting
Monoclonal Antibodies
milk fat globule

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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Human milk fat globule membrane glycoproteins express blood group-related determinants primarily on mucin-like epithelial membrane antigens and gp70. / Dion, A. S.; Williams, Charlene; Herlyn, M.; Major, P.

In: Biochemistry International, Vol. 22, No. 2, 01.12.1990, p. 295-302.

Research output: Contribution to journalArticle

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