H-DNA and Z-DNA in the mouse c-Ki-ras promoter

Dmitri G. Pestov, Andrey Dayn, Elena Yu Siyanova, Donna L. George, Sergei M. Mirkin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, chloroacetaldehyde, and dimethyl sulfate. The results demonstrate that two types of unusual DNA structures formed under different environmental conditions. A 27-bp homopurinehomopyrimidine mirror repeat adopts a triple-helical HDNA conformation under mildly acidic conditions. This H-DNA seems to account for the S1 hypersensitivity of the promoter in vitro, since the observed pattern of S1 hypersensitivity at a single base level fits well with the H-DNA formation. Under conditions of neutral pH we have detected Z-DNA created by a (CG)5-stretch, located adjacent to the homopurine-homopyrimidine mirror repeat. The ability of the promoter DNA segment to form non-B structures has implications for models of gene regulation.

Original languageEnglish (US)
Pages (from-to)6527-6532
Number of pages6
JournalNucleic acids research
Volume19
Issue number23
DOIs
StatePublished - Dec 11 1991

Fingerprint

Z-Form DNA
Promoter
Mouse
DNA
Conformation
Mirror
Hypersensitivity
Diethyl Pyrocarbonate
Osmium Tetroxide
Nucleic Acid Conformation
Conformations
Mirrors
Gene Regulation
Stretch
Probe
Adjacent
Gene expression
Osmium
Demonstrate
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Pestov, D. G., Dayn, A., Siyanova, E. Y., George, D. L., & Mirkin, S. M. (1991). H-DNA and Z-DNA in the mouse c-Ki-ras promoter. Nucleic acids research, 19(23), 6527-6532. https://doi.org/10.1093/nar/19.23.6527
Pestov, Dmitri G. ; Dayn, Andrey ; Siyanova, Elena Yu ; George, Donna L. ; Mirkin, Sergei M. / H-DNA and Z-DNA in the mouse c-Ki-ras promoter. In: Nucleic acids research. 1991 ; Vol. 19, No. 23. pp. 6527-6532.
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Pestov, DG, Dayn, A, Siyanova, EY, George, DL & Mirkin, SM 1991, 'H-DNA and Z-DNA in the mouse c-Ki-ras promoter', Nucleic acids research, vol. 19, no. 23, pp. 6527-6532. https://doi.org/10.1093/nar/19.23.6527

H-DNA and Z-DNA in the mouse c-Ki-ras promoter. / Pestov, Dmitri G.; Dayn, Andrey; Siyanova, Elena Yu; George, Donna L.; Mirkin, Sergei M.

In: Nucleic acids research, Vol. 19, No. 23, 11.12.1991, p. 6527-6532.

Research output: Contribution to journalArticle

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Pestov DG, Dayn A, Siyanova EY, George DL, Mirkin SM. H-DNA and Z-DNA in the mouse c-Ki-ras promoter. Nucleic acids research. 1991 Dec 11;19(23):6527-6532. https://doi.org/10.1093/nar/19.23.6527