Global alterations of the transcriptional landscape during yeast growth and development in the absence of Ume6-dependent chromatin modification

Aurélie Lardenois, Emmanuelle Becker, Thomas Walther, Michael J. Law, Bingning Xie, Philippe Demougin, Randy Strich, Michael Primig

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Chromatin modification enzymes are important regulators of gene expression and some are evolutionarily conserved from yeast to human. Saccharomyces cerevisiae is a major model organism for genome-wide studies that aim at the identification of target genes under the control of conserved epigenetic regulators. Ume6 interacts with the upstream repressor site 1 (URS1) and represses transcription by recruiting both the conserved histone deacetylase Rpd3 (through the co-repressor Sin3) and the chromatin-remodeling factor Isw2. Cells lacking Ume6 are defective in growth, stress response, and meiotic development. RNA profiling studies and in vivo protein-DNA binding assays identified mRNAs or transcript isoforms that are directly repressed by Ume6 in mitosis. However, a comprehensive understanding of the transcriptional alterations, which underlie the complex ume6Δ mutant phenotype during fermentation, respiration, or sporulation, is lacking. We report the protein-coding transcriptome of a diploid MATa/α wild-type and ume6/ume6 mutant strains cultured in rich media with glucose or acetate as a carbon source, or sporulation-inducing medium. We distinguished direct from indirect effects on mRNA levels by combining GeneChip data with URS1 motif predictions and published high-throughput in vivo Ume6-DNA binding data. To gain insight into the molecular interactions between successive waves of Ume6-dependent meiotic genes, we integrated expression data with information on protein networks. Our work identifies novel Ume6 repressed genes during growth and development and reveals a strong effect of the carbon source on the derepression pattern of transcripts in growing and developmentally arrested ume6/ume6 mutant cells. Since yeast is a useful model organism for chromatin-mediated effects on gene expression, our results provide a rich source for further genetic and molecular biological work on the regulation of cell growth and cell differentiation in eukaryotes.

Original languageEnglish (US)
Pages (from-to)2031-2046
Number of pages16
JournalMolecular Genetics and Genomics
Volume290
Issue number5
DOIs
StatePublished - Oct 23 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Global alterations of the transcriptional landscape during yeast growth and development in the absence of Ume6-dependent chromatin modification'. Together they form a unique fingerprint.

Cite this