Genetic selection of growth-inhibitory sequences in mammalian cells

Dmitri G. Pestov, Lester F. Lau

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44(MAPK) that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.

Original languageEnglish (US)
Pages (from-to)12549-12553
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number26
StatePublished - Dec 20 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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