Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules

Miao Lu; Dongjin Lee; Wei Xue; Tianhong Cui

doi:10.1109/MEMSYS.2008.4443624

Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules

Miao Lu, Dongjin Lee, Wei Xue, Tianhong Cui

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNT) and biomolecules are self-assembled on micro patterned electrodes. The immuno-chip acts as the platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. The detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring, etc.

Original language	English (US)
Title of host publication	MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages	188-191
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2008.4443624
State	Published - 2008
Externally published	Yes
Event	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States Duration: Jan 13 2008 → Jan 17 2008

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
Country/Territory	United States
City	Tucson, AZ
Period	1/13/08 → 1/17/08

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2008.4443624

Cite this

Lu, M., Lee, D., Xue, W., & Cui, T. (2008). Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules. In MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems (pp. 188-191). Article 4443624 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2008.4443624

@inproceedings{bc4e9444c06446afbf2a52fc0864d860,

title = "Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules",

abstract = "A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNT) and biomolecules are self-assembled on micro patterned electrodes. The immuno-chip acts as the platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. The detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring, etc.",

author = "Miao Lu and Dongjin Lee and Wei Xue and Tianhong Cui",

note = "Funding Information: This work is partially supported by DARPA MEMS/NEMS Fundamental Research Program through the MF3 Center. ; 21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson ; Conference date: 13-01-2008 Through 17-01-2008",

year = "2008",

doi = "10.1109/MEMSYS.2008.4443624",

language = "English (US)",

isbn = "9781424417933",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "188--191",

booktitle = "MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems",

}

Lu, M, Lee, D, Xue, W & Cui, T 2008, Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules. in MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems., 4443624, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 188-191, 21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson, Tucson, AZ, United States, 1/13/08. https://doi.org/10.1109/MEMSYS.2008.4443624

Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules. / Lu, Miao; Lee, Dongjin; Xue, Wei et al.
MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems. 2008. p. 188-191 4443624 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules

AU - Lu, Miao

AU - Lee, Dongjin

AU - Xue, Wei

AU - Cui, Tianhong

N1 - Funding Information: This work is partially supported by DARPA MEMS/NEMS Fundamental Research Program through the MF3 Center.

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N2 - A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNT) and biomolecules are self-assembled on micro patterned electrodes. The immuno-chip acts as the platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. The detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring, etc.

AB - A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNT) and biomolecules are self-assembled on micro patterned electrodes. The immuno-chip acts as the platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. The detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring, etc.

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems

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Lu M, Lee D, Xue W, Cui T. Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules. In MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems. 2008. p. 188-191. 4443624. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2008.4443624

Flexible and disposable immunosensors based on layer-bylayer self-assembled carbon nanotubes and biomolecules

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