Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

Irina Dimitrova; Garabet G. Toby; Esmerina Tili; Randy Strich; Sotirios C. Kampranis; Antonios M. Makris

doi:10.1016/j.febslet.2004.04.012

Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

Irina Dimitrova, Garabet G. Toby, Esmerina Tili, Randy Strich, Sotirios C. Kampranis, Antonios M. Makris

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11 Scopus citations

Abstract

Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

Original language	English (US)
Pages (from-to)	100-104
Number of pages	5
Journal	FEBS Letters
Volume	566
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.04.012
State	Published - May 21 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.04.012

Cite this

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title = "Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic",

abstract = "Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.",

author = "Irina Dimitrova and Toby, {Garabet G.} and Esmerina Tili and Randy Strich and Kampranis, {Sotirios C.} and Makris, {Antonios M.}",

year = "2004",

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TY - JOUR

T1 - Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

AU - Dimitrova, Irina

AU - Toby, Garabet G.

AU - Tili, Esmerina

AU - Strich, Randy

AU - Kampranis, Sotirios C.

AU - Makris, Antonios M.

PY - 2004/5/21

Y1 - 2004/5/21

N2 - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

AB - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

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Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

Abstract

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