TY - JOUR
T1 - Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic
AU - Dimitrova, Irina
AU - Toby, Garabet G.
AU - Tili, Esmerina
AU - Strich, Randy
AU - Kampranis, Sotirios C.
AU - Makris, Antonios M.
N1 - Funding Information:
We thank Dr. B. Westermann for providing us with plasmid pVT100U-mitGFP and Dr. S.D. Emr for the GFP-ALP fusion construct. This work was supported by the EU DGI-AIDCO/CIHEAM-COMBINE program.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/5/21
Y1 - 2004/5/21
N2 - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.
AB - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.
UR - http://www.scopus.com/inward/record.url?scp=2442479702&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442479702&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.04.012
DO - 10.1016/j.febslet.2004.04.012
M3 - Article
C2 - 15147876
AN - SCOPUS:2442479702
VL - 566
SP - 100
EP - 104
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -