Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

Irina Dimitrova, Garabet G. Toby, Esmerina Tili, Randy Strich, Sotirios C. Kampranis, Antonios M. Makris

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

Original languageEnglish (US)
Pages (from-to)100-104
Number of pages5
JournalFEBS Letters
Volume566
Issue number1-3
DOIs
StatePublished - May 21 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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