Exposure to CuO nanoparticles mediates NFκB activation and enhances amyloid precursor protein expression

Xiaoyang Mou, Alexander Pilozzi, Breeya Tailor, Jing Yi, Catherine Cahill, Jack Rogers, Xudong Huang

Research output: Contribution to journalArticle

Abstract

Amyloid precursor protein (APP) is directly related to Aβ amyloidosis-a hallmark of Alzheimer's disease (AD). However, the impact of environmental factors upon APP biology and Aβ amyloid pathology have not been well studied. The increased use of nanoparticles (NPs) or engineered nanomaterials (ENMs) has led to a growing body of evidence suggesting that exposure to metal/metal oxide NPs, such as Fe2O3, CuO, and ZnO, may contribute to the pathophysiology of neurodegenerative diseases such as AD through neuroinflammation. Our previous studies indicated that exposure to CuO nanoparticles (CuONPs) induce potent in vitro neurotoxicity. Herein, we investigated the effects on APP expression in neuronal cells exposed to different metal oxide NPs. We found a low dose of CuONPs effectively activated the NFκB signaling pathway and increased APP expression. Moreover, the inhibition of p65 expression using siRNA abolished CuONP-mediated APP expression, suggesting that NFκB-regulated APP expression in response to CuONP exposure may be associated with AD pathology.

Original languageEnglish (US)
Article number45
JournalBiomedicines
Volume8
Issue number3
DOIs
StatePublished - Mar 1 2020

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this