Evidence for paracrine signaling between macrophages and bovine adrenal chromaffin cell Ca2+ channels

Kevin P.M. Currie, Zhong Zhou, Fox Aaron P

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The adrenal gland contains resident macrophages, some of which lie adjacent to the catecholamine producing chromaffin cells. Because macrophages release a variety of secretory products, it is possible that paracrine signaling between these two cell types exists. Of particular interest is the potential paracrine modulation of voltage-gated calcium channels (I(Ca)), which are the main calcium influx pathway triggering catecholamine release from chromaffin cells. We report that prostaglandin E2 (PGE2), one of the main signals produced by macrophages, inhibited I(Ca) in cultured bovine adrenal chromaffin cells. The inhibition is rapid, robust, and voltage dependent; the activation kinetics are slowed and inhibition is largely reversed by a large depolarizing prepulse, suggesting that the inhibition is mediated by a direct G-protein βγ subunit interaction with the calcium channels. About half of the response to PGE2 was sensitive to pertussis toxin (PTX) incubation, suggesting both PTX-sensitive and -insensitive G proteins were involved. We show that activation of macrophages by endotoxin rapidly (within minutes) releases a signal that inhibits I(Ca) in chromaffin cells. The inhibition is voltage dependent and partially PTX sensitive. PGE2 is not responsible for this inhibition as blocking cyclooxygenase with ibuprofen did not prevent the production of the inhibitory signal by the macrophages. Nor did blocking the lipoxy-genase pathway with nordihydroguaiaretic acid alter production of the inhibitory signal. Our results suggest that macrophages may modulate I(Ca) and catecholamine secretion by releasing PGE2 and other chemical signal(s).

Original languageEnglish (US)
Pages (from-to)280-287
Number of pages8
JournalJournal of Neurophysiology
Volume83
Issue number1
StatePublished - Jan 20 2000

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Paracrine Communication
Chromaffin Cells
Dinoprostone
Macrophages
Pertussis Toxin
Catecholamines
Calcium Channels
GTP-Binding Proteins
Masoprocol
Macrophage Activation
Ibuprofen
Protein Subunits
Prostaglandin-Endoperoxide Synthases
Adrenal Glands
Endotoxins
Calcium

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

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title = "Evidence for paracrine signaling between macrophages and bovine adrenal chromaffin cell Ca2+ channels",
abstract = "The adrenal gland contains resident macrophages, some of which lie adjacent to the catecholamine producing chromaffin cells. Because macrophages release a variety of secretory products, it is possible that paracrine signaling between these two cell types exists. Of particular interest is the potential paracrine modulation of voltage-gated calcium channels (I(Ca)), which are the main calcium influx pathway triggering catecholamine release from chromaffin cells. We report that prostaglandin E2 (PGE2), one of the main signals produced by macrophages, inhibited I(Ca) in cultured bovine adrenal chromaffin cells. The inhibition is rapid, robust, and voltage dependent; the activation kinetics are slowed and inhibition is largely reversed by a large depolarizing prepulse, suggesting that the inhibition is mediated by a direct G-protein βγ subunit interaction with the calcium channels. About half of the response to PGE2 was sensitive to pertussis toxin (PTX) incubation, suggesting both PTX-sensitive and -insensitive G proteins were involved. We show that activation of macrophages by endotoxin rapidly (within minutes) releases a signal that inhibits I(Ca) in chromaffin cells. The inhibition is voltage dependent and partially PTX sensitive. PGE2 is not responsible for this inhibition as blocking cyclooxygenase with ibuprofen did not prevent the production of the inhibitory signal by the macrophages. Nor did blocking the lipoxy-genase pathway with nordihydroguaiaretic acid alter production of the inhibitory signal. Our results suggest that macrophages may modulate I(Ca) and catecholamine secretion by releasing PGE2 and other chemical signal(s).",
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Evidence for paracrine signaling between macrophages and bovine adrenal chromaffin cell Ca2+ channels. / Currie, Kevin P.M.; Zhou, Zhong; Aaron P, Fox.

In: Journal of Neurophysiology, Vol. 83, No. 1, 20.01.2000, p. 280-287.

Research output: Contribution to journalArticle

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