Evidence for a regional specificity in the density and distribution of noradrenergic varicosities in rat cortex

Kara L. Agster, Carlos A. Mejias-Aponte, Brian D. Clark, Barry Waterhouse

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The brainstem nucleus locus coeruleus (LC) is the sole source of norepinephrine (NE)-containing fibers in the mammalian cortex. Previous studies suggest that the density of noradrenergic fibers in rat is relatively uniform across cortical regions and that cells in the nucleus discharge en masse. This implies that activation of the LC results in equivalent release of NE throughout the cortex. However, it is possible that there could be differences in the density of axonal varicosities across regions, and that these differences, rather than a difference in fiber density, may contribute to the regulation of NE efflux. Quantification of dopamine β-hydroxylase (DβH)-immunostained varicosities was performed on several cortical regions and in the ventral posterior medial (VPM) thalamus by using unbiased sampling methods. The density of DβH varicosities is greater in the prefrontal cortex than in motor, somatosensory, or piriform cortices, greater in superficial than in deep layers of cortex, and greater in the VPM than in the somatosensory cortex. Our results provide anatomical evidence for non-uniform release of NE across functionally discrete cortical regions. This morphology may account for a differential, region-specific, impact of LC output on different cortical areas.

Original languageEnglish (US)
Pages (from-to)2195-2207
Number of pages13
JournalJournal of Comparative Neurology
Volume521
Issue number10
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Fingerprint

Locus Coeruleus
Norepinephrine
Somatosensory Cortex
Mixed Function Oxygenases
Dopamine
Motor Cortex
Prefrontal Cortex
Cell Nucleus
Thalamus
Brain Stem

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Agster, Kara L. ; Mejias-Aponte, Carlos A. ; Clark, Brian D. ; Waterhouse, Barry. / Evidence for a regional specificity in the density and distribution of noradrenergic varicosities in rat cortex. In: Journal of Comparative Neurology. 2013 ; Vol. 521, No. 10. pp. 2195-2207.
@article{4e2e9c9cd396424da1490522352391d0,
title = "Evidence for a regional specificity in the density and distribution of noradrenergic varicosities in rat cortex",
abstract = "The brainstem nucleus locus coeruleus (LC) is the sole source of norepinephrine (NE)-containing fibers in the mammalian cortex. Previous studies suggest that the density of noradrenergic fibers in rat is relatively uniform across cortical regions and that cells in the nucleus discharge en masse. This implies that activation of the LC results in equivalent release of NE throughout the cortex. However, it is possible that there could be differences in the density of axonal varicosities across regions, and that these differences, rather than a difference in fiber density, may contribute to the regulation of NE efflux. Quantification of dopamine β-hydroxylase (DβH)-immunostained varicosities was performed on several cortical regions and in the ventral posterior medial (VPM) thalamus by using unbiased sampling methods. The density of DβH varicosities is greater in the prefrontal cortex than in motor, somatosensory, or piriform cortices, greater in superficial than in deep layers of cortex, and greater in the VPM than in the somatosensory cortex. Our results provide anatomical evidence for non-uniform release of NE across functionally discrete cortical regions. This morphology may account for a differential, region-specific, impact of LC output on different cortical areas.",
author = "Agster, {Kara L.} and Mejias-Aponte, {Carlos A.} and Clark, {Brian D.} and Barry Waterhouse",
year = "2013",
month = "7",
day = "1",
doi = "10.1002/cne.23270",
language = "English (US)",
volume = "521",
pages = "2195--2207",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "10",

}

Evidence for a regional specificity in the density and distribution of noradrenergic varicosities in rat cortex. / Agster, Kara L.; Mejias-Aponte, Carlos A.; Clark, Brian D.; Waterhouse, Barry.

In: Journal of Comparative Neurology, Vol. 521, No. 10, 01.07.2013, p. 2195-2207.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evidence for a regional specificity in the density and distribution of noradrenergic varicosities in rat cortex

AU - Agster, Kara L.

AU - Mejias-Aponte, Carlos A.

AU - Clark, Brian D.

AU - Waterhouse, Barry

PY - 2013/7/1

Y1 - 2013/7/1

N2 - The brainstem nucleus locus coeruleus (LC) is the sole source of norepinephrine (NE)-containing fibers in the mammalian cortex. Previous studies suggest that the density of noradrenergic fibers in rat is relatively uniform across cortical regions and that cells in the nucleus discharge en masse. This implies that activation of the LC results in equivalent release of NE throughout the cortex. However, it is possible that there could be differences in the density of axonal varicosities across regions, and that these differences, rather than a difference in fiber density, may contribute to the regulation of NE efflux. Quantification of dopamine β-hydroxylase (DβH)-immunostained varicosities was performed on several cortical regions and in the ventral posterior medial (VPM) thalamus by using unbiased sampling methods. The density of DβH varicosities is greater in the prefrontal cortex than in motor, somatosensory, or piriform cortices, greater in superficial than in deep layers of cortex, and greater in the VPM than in the somatosensory cortex. Our results provide anatomical evidence for non-uniform release of NE across functionally discrete cortical regions. This morphology may account for a differential, region-specific, impact of LC output on different cortical areas.

AB - The brainstem nucleus locus coeruleus (LC) is the sole source of norepinephrine (NE)-containing fibers in the mammalian cortex. Previous studies suggest that the density of noradrenergic fibers in rat is relatively uniform across cortical regions and that cells in the nucleus discharge en masse. This implies that activation of the LC results in equivalent release of NE throughout the cortex. However, it is possible that there could be differences in the density of axonal varicosities across regions, and that these differences, rather than a difference in fiber density, may contribute to the regulation of NE efflux. Quantification of dopamine β-hydroxylase (DβH)-immunostained varicosities was performed on several cortical regions and in the ventral posterior medial (VPM) thalamus by using unbiased sampling methods. The density of DβH varicosities is greater in the prefrontal cortex than in motor, somatosensory, or piriform cortices, greater in superficial than in deep layers of cortex, and greater in the VPM than in the somatosensory cortex. Our results provide anatomical evidence for non-uniform release of NE across functionally discrete cortical regions. This morphology may account for a differential, region-specific, impact of LC output on different cortical areas.

UR - http://www.scopus.com/inward/record.url?scp=84876740290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876740290&partnerID=8YFLogxK

U2 - 10.1002/cne.23270

DO - 10.1002/cne.23270

M3 - Article

C2 - 23184811

AN - SCOPUS:84876740290

VL - 521

SP - 2195

EP - 2207

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 10

ER -