Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta

Dania V. Rossi, Ying Dai, Peter Thomas, Gonzalo A. Carrasco, Lydia L. DonCarlos, Nancy A. Muma, Qian Li

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Estradiol regulates serotonin 1A (5-HT1A) receptor signaling. Since desensitization of 5-HT1A receptors may be an underlying mechanism by which selective serotonin reuptake inhibitors (SSRIs) mediate their therapeutic effects and combining estradiol with SSRIs enhances the efficacy of the SSRIs, it is important to determine which estrogen receptors are capable of desensitizating 5-HT1A receptor function. We previously demonstrated that selective activation of the estrogen receptor, GPR30, desensitizes 5-HT1A receptor signaling in rat hypothalamic paraventricular nucleus (PVN). However, since estrogen receptor-beta (ERβ), is highly expressed in the PVN, we investigated the role of ERβ in estradiol-induced desensitization of 5-HT1A receptor signaling. We first showed that a selective ERβ agonist, diarylpropionitrile (DPN) has a 100-fold lower binding affinity than estradiol for GPR30. Administration of DPN did not desensitize 5-HT1A receptor signaling in rat PVN as demonstrated by agonist-stimulated hormone release. Second, we used a recombinant adenovirus containing ERβ siRNAs to decrease ERβ expression in the PVN. Reductions in ERβ did not alter the estradiol-induced desensitization of 5-HT1A receptor signaling in oxytocin cells. In contrast, in animals with reduced ERβ, estradiol administration, instead of producing desensitization, augmented the ACTH response to a 5-HT1A agonist. Combined with the results from the DPN treatment experiments, desensitization of 5-HT1A receptor signaling does not appear to be mediated by ERβ in oxytocin cells, but that ERβ, together with GPR30, may play a complex role in central regulation of 5-HT1A-mediated ACTH release. Determining the mechanisms by which estrogens induce desensitization may aid in the development of better treatments for mood disorders.

Original languageEnglish (US)
Pages (from-to)1023-1033
Number of pages11
JournalPsychoneuroendocrinology
Volume35
Issue number7
DOIs
StatePublished - Aug 1 2010

Fingerprint

Estrogen Receptor beta
Receptor, Serotonin, 5-HT1A
Paraventricular Hypothalamic Nucleus
Hypothalamus
Estradiol
Serotonin Uptake Inhibitors
Oxytocin
Estrogen Receptors
Adrenocorticotropic Hormone
Estrogens
Serotonin 5-HT1 Receptor Agonists
Therapeutic Uses
Mood Disorders
Adenoviridae
Psychologic Desensitization
Hormones

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Biological Psychiatry

Cite this

Rossi, Dania V. ; Dai, Ying ; Thomas, Peter ; Carrasco, Gonzalo A. ; DonCarlos, Lydia L. ; Muma, Nancy A. ; Li, Qian. / Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta. In: Psychoneuroendocrinology. 2010 ; Vol. 35, No. 7. pp. 1023-1033.
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abstract = "Estradiol regulates serotonin 1A (5-HT1A) receptor signaling. Since desensitization of 5-HT1A receptors may be an underlying mechanism by which selective serotonin reuptake inhibitors (SSRIs) mediate their therapeutic effects and combining estradiol with SSRIs enhances the efficacy of the SSRIs, it is important to determine which estrogen receptors are capable of desensitizating 5-HT1A receptor function. We previously demonstrated that selective activation of the estrogen receptor, GPR30, desensitizes 5-HT1A receptor signaling in rat hypothalamic paraventricular nucleus (PVN). However, since estrogen receptor-beta (ERβ), is highly expressed in the PVN, we investigated the role of ERβ in estradiol-induced desensitization of 5-HT1A receptor signaling. We first showed that a selective ERβ agonist, diarylpropionitrile (DPN) has a 100-fold lower binding affinity than estradiol for GPR30. Administration of DPN did not desensitize 5-HT1A receptor signaling in rat PVN as demonstrated by agonist-stimulated hormone release. Second, we used a recombinant adenovirus containing ERβ siRNAs to decrease ERβ expression in the PVN. Reductions in ERβ did not alter the estradiol-induced desensitization of 5-HT1A receptor signaling in oxytocin cells. In contrast, in animals with reduced ERβ, estradiol administration, instead of producing desensitization, augmented the ACTH response to a 5-HT1A agonist. Combined with the results from the DPN treatment experiments, desensitization of 5-HT1A receptor signaling does not appear to be mediated by ERβ in oxytocin cells, but that ERβ, together with GPR30, may play a complex role in central regulation of 5-HT1A-mediated ACTH release. Determining the mechanisms by which estrogens induce desensitization may aid in the development of better treatments for mood disorders.",
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Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta. / Rossi, Dania V.; Dai, Ying; Thomas, Peter; Carrasco, Gonzalo A.; DonCarlos, Lydia L.; Muma, Nancy A.; Li, Qian.

In: Psychoneuroendocrinology, Vol. 35, No. 7, 01.08.2010, p. 1023-1033.

Research output: Contribution to journalArticle

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AU - Rossi, Dania V.

AU - Dai, Ying

AU - Thomas, Peter

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AU - Li, Qian

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