Enzyme-linked immunosorbent assay to quantify targeting molecules on nanoparticles

Rachel S. Riley, Jilian R. Melamed, Emily S. Day

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations


Molecular targeting presents a promising means of improving the specificity of cancer therapeutics, increasing accumulation at the cancer site and limiting off-target effects. These targeting schemes can be applied to nanoparticle-based treatments to further enhance their anticancer efficacy. Here, we describe methods to conjugate antibodies to silica-gold nanoshells and to quantify the resulting antibody content on the nanoparticles using a solution-based enzyme-linked immunosorbent assay (ELISA). Although we will be using anti-EGFR (epidermal growth factor receptor) antibodies conjugated to gold-silica nanoshells as a model system, this method is adaptable to quantify a range of targeting antibodies and proteins on various types of nanoparticles.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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