Embryonic stem cell-specific microRNAs

Hristo B. Houbaviy; Michael F. Murray; Phillip A. Sharp

doi:10.1016/S1534-5807(03)00227-2

Embryonic stem cell-specific microRNAs

Hristo B. Houbaviy, Michael F. Murray, Phillip A. Sharp

Research output: Contribution to journal › Article › peer-review

977 Scopus citations

Abstract

We have identified microRNAs (miRNAs) in undifferentiated and differentiated mouse embryonic stem (ES) cells. Some of these appear to be ES cell specific, have related sequences, and are encoded by genomic loci clustered within 2.2 kb of each other. Their expression is repressed as ES cells differentiate into embryoid bodies and is undetectable in adult mouse organs. In contrast, the levels of many previously described miRNAs remain constant or increase upon differentiation. Our results suggest that miRNAs may have a role in the maintenance of the pluripotent cell state and in the regulation of early mammalian development.

Original language	English (US)
Pages (from-to)	351-358
Number of pages	8
Journal	Developmental Cell
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/S1534-5807(03)00227-2
State	Published - Aug 1 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/S1534-5807(03)00227-2

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AU - Sharp, Phillip A.

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AB - We have identified microRNAs (miRNAs) in undifferentiated and differentiated mouse embryonic stem (ES) cells. Some of these appear to be ES cell specific, have related sequences, and are encoded by genomic loci clustered within 2.2 kb of each other. Their expression is repressed as ES cells differentiate into embryoid bodies and is undetectable in adult mouse organs. In contrast, the levels of many previously described miRNAs remain constant or increase upon differentiation. Our results suggest that miRNAs may have a role in the maintenance of the pluripotent cell state and in the regulation of early mammalian development.

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Embryonic stem cell-specific microRNAs

Abstract

All Science Journal Classification (ASJC) codes

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