Electrochemical redundant microsensor arrays for glucose monitoring with patterned polymer films

Redundant microsensor arrays for glucose sensing were fabricated using photopolymerization of poly(ethylene glycol) diacrylate (PEG-DA) with 2-hydroxy-2-methyl phenyl-propanone as photoinitiator to encapsulate the enzyme glucose oxidase. Silicon micro fabrication technologies were used to fabricate microelectrode sensor arrays on flexible polyimide sheets. These microarray sensors were individually addressable as observed using square-wave voltammetry. Redox polymer, poly[4-vinylpyridine Os(bipyridine)₂Cl]-co- ethylamine, was first immobilized on the electrode surface and then glucose oxidase was entrapped in PEG-DA hydrogels. The redox polymer was found to exchange electrons with glucose oxidase in biocompatible PEG-DA hydrogels. The entrapped glucose oxidase was found to respond linearly to glucose in solution (0-20 mM) as determined using square-wave voltammetry.