Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Z. Mazzochette, E. Newton, A. Mugweru

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM-1 sensitivity. The calculated apparent Michaelis-Menten constant Km for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.

Original languageEnglish (US)
Pages (from-to)2997-3002
Number of pages6
JournalAnalytical Methods
Volume9
Issue number20
DOIs
StatePublished - May 28 2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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