Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Z. Mazzochette; E. Newton; A. Mugweru

doi:10.1039/c7ay00431a

Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Z. Mazzochette, E. Newton, A. Mugweru

Chemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM^-1 sensitivity. The calculated apparent Michaelis-Menten constant K_m for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.

Original language	English (US)
Pages (from-to)	2997-3002
Number of pages	6
Journal	Analytical Methods
Volume	9
Issue number	20
DOIs	https://doi.org/10.1039/c7ay00431a
State	Published - May 28 2017

All Science Journal Classification (ASJC) codes

Analytical Chemistry
General Chemical Engineering
General Engineering

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10.1039/c7ay00431a

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title = "Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers",

abstract = "Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM-1 sensitivity. The calculated apparent Michaelis-Menten constant Km for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.",

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AU - Mazzochette, Z.

AU - Newton, E.

AU - Mugweru, A.

PY - 2017/5/28

Y1 - 2017/5/28

N2 - Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM-1 sensitivity. The calculated apparent Michaelis-Menten constant Km for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.

AB - Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM-1 sensitivity. The calculated apparent Michaelis-Menten constant Km for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.

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Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Abstract

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