Effects of lipoxin A4 on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine

O. Soyombo; B. W. Spur; T. H. Lee

doi:10.1111/j.1398-9995.1994.tb02654.x

Effects of lipoxin A₄ on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine

O. Soyombo, B. W. Spur, T. H. Lee

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A₄ (LXA₄) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA₄ is a weak and partial chemotactic agent; at 10⁻⁶ M, it achieved about 20% of the response of 10⁻⁶ M platelet‐activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA₄ (10⁻¹⁰−10⁻⁵ M) resulted in a concentration‐dependent inhibition of cell migration induced by 10^‐6 M formyl‐methionyl‐leucyl‐phenylalanine (FMLP) and 10^‐6 M PAF. The concentration of LXA₄ which produced 50% inhibition (IC₅₀) of eosinophil migration was approximately 10^‐6 M. LXA₄ (10^‐10‐10^‐6 M) did not elicit ECP release or modulate ECP release induced by 10^‐6 M FMLP. LXA₄ may have antiallergic properties in preventing eosinophilic migration.

Original language	English (US)
Pages (from-to)	230-234
Number of pages	5
Journal	Acta Allergologica
Volume	49
Issue number	4
DOIs	https://doi.org/10.1111/j.1398-9995.1994.tb02654.x
State	Published - Apr 1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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title = "Effects of lipoxin A4 on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine",

abstract = "Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10−6 M, it achieved about 20% of the response of 10−6 M platelet‐activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10−10−10−5 M) resulted in a concentration‐dependent inhibition of cell migration induced by 10‐6 M formyl‐methionyl‐leucyl‐phenylalanine (FMLP) and 10‐6 M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10‐6 M. LXA4 (10‐10‐10‐6 M) did not elicit ECP release or modulate ECP release induced by 10‐6 M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.",

author = "O. Soyombo and Spur, {B. W.} and Lee, {T. H.}",

year = "1994",

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doi = "10.1111/j.1398-9995.1994.tb02654.x",

language = "English (US)",

volume = "49",

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journal = "Acta Allergologica",

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T1 - Effects of lipoxin A4 on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine

AU - Soyombo, O.

AU - Spur, B. W.

AU - Lee, T. H.

PY - 1994/4

Y1 - 1994/4

N2 - Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10−6 M, it achieved about 20% of the response of 10−6 M platelet‐activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10−10−10−5 M) resulted in a concentration‐dependent inhibition of cell migration induced by 10‐6 M formyl‐methionyl‐leucyl‐phenylalanine (FMLP) and 10‐6 M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10‐6 M. LXA4 (10‐10‐10‐6 M) did not elicit ECP release or modulate ECP release induced by 10‐6 M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.

AB - Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10−6 M, it achieved about 20% of the response of 10−6 M platelet‐activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10−10−10−5 M) resulted in a concentration‐dependent inhibition of cell migration induced by 10‐6 M formyl‐methionyl‐leucyl‐phenylalanine (FMLP) and 10‐6 M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10‐6 M. LXA4 (10‐10‐10‐6 M) did not elicit ECP release or modulate ECP release induced by 10‐6 M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.

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SN - 0105-4538

VL - 49

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JO - Acta Allergologica

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Effects of lipoxin A₄ on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine

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