Dose-dependent outcome of ebv infection of humanized mice based on green raji unit (Gru) doses

Haiwen Chen, Ling Zhong, Wanlin Zhang, Shanshan Zhang, Junping Hong, Xiang Zhou, Xinyu Zhang, Qisheng Feng, Yixin Chen, Yi Xin Zeng, Miao Xu, Claude Krummenacher, Xiao Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Humanized mouse models are used as comprehensive small-animal models of EBV infec-tion. Previously, infectious doses of EBV used in vivo have been determined mainly on the basis of TD50 (50% transforming dose), which is a time-consuming process. Here, we determined infectious doses of Akata-EBV-GFP using green Raji units (GRUs), and characterized dose-dependent effects in humanized mice. We defined two outcomes in vivo, including an infection model and a lymphoma model, following inoculation with low or high doses of Akata-EBV-GFP, respectively. Inoculation with a low dose induced primary B cells to become lymphoblastoid cell lines in vitro, and caused latent infection in humanized mice. In contrast, a high dose of Akata-EBV-GFP resulted in primary B cells death in vitro, and fatal B cell lymphomas in vivo. Following infection with high doses, the frequency of CD19+ B cells decreased, whereas the percentage of CD8+ T cells increased in peripheral blood and the spleen. At such doses, a small part of activated CD8+ T cells was EBV-specific CD8+ T cells. Thus, GRUs quantitation of Akata-EBV-GFP is an effective way to quantify infectious doses to study pathologies, immune response, and to assess (in vivo) the neutralizing activity of antibodies raised by immunization against EBV.

Original languageEnglish (US)
Article number2184
JournalViruses
Volume13
Issue number11
DOIs
StatePublished - Nov 2021

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

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