Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations

Frederic F. Depreux, Megan J. Puckelwartz, Aleksandra Augustynowicz, Don Wolfgeher, Christine M. Labno, Dynora Pierre-Louis, Danielle Cicka, Stephen J. Kron, James Holaska, Elizabeth M. McNally

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The nuclear face of the nuclear membrane is enriched with the intermediate filament protein lamin A. Mutations in LMNA, the gene encoding lamin A, lead to a diverse set of inherited conditions including myopathies that affect both the heart and skeletal muscle. To gain insight about lamin A protein interactions, binding proteins associated with the tail of lamin A were characterized. Of 130 nuclear proteins found associated with the lamin A tail, 17 (13%) were previously described lamin A binding partners. One protein not previously linked to lamin A, matrin-3, was selected for further study, because like LMNA mutations, matrin-3 has also been implicated in inherited myopathy. Matrin-3 binds RNA and DNA and is a nucleoplasmic protein originally identified from the insoluble nuclear fraction, referred to as the nuclear matrix. Anti-matrin-3 antibodies were found to co-immunoprecipitate lamin A, and the lamin-A binding domain was mapped to the carboxy-terminal half of matrin-3. Three-dimensional mapping of the lamin A-matrin-3 interface showed that the LMNA truncating mutation Δ303, which lacks the matrin-3 binding domain, was associated with an increased distance between lamin A and matrin-3. LMNA mutant cells are known to have altered biophysical properties and the matrin-3–lamin A interface is positioned to contribute to these defects.

Original languageEnglish (US)
Pages (from-to)4284-4295
Number of pages12
JournalHuman molecular genetics
Volume24
Issue number15
DOIs
StatePublished - Aug 1 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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