Differential cellular composition of human palatine and pharyngeal tonsils

Luke Stanisce, Etty Sims, Cheryl Hou, Yekaterina Koshkareva, John P. Gaughan, Igor Kuzin, Andrea Bottaro

Research output: Contribution to journalArticle

Abstract

Objective: The goal of this study was to gain a better understanding of the potential functional specialization of palatine and pharyngeal tonsils, by comparing their cellular composition in paired specimens from a large cohort of adenotonsillectomy patients. Design: Resident B cell, T cell, dendritic cell, and stromal cell subsets were characterized using multicolor flow cytometry in palatine and pharyngeal tonsil specimens from 27 patients, age 2–34 years. Results: Paired comparisons showed highly significant intra-individual differences in resident cell subsets of palatine and pharyngeal tonsils. Palatine tonsils harbored higher fractions of germinal center B cells/plasmablasts and IgD- CD27- double-negative B cells, and conversely lower fractions of IgD + CD38- resting naïve B cells compared to pharyngeal tonsils. Palatine tonsils also showed lower fractions of plasmacytoid dendritic cells, and higher percentages of two subsets of stromal cells - fibroblastic reticular cells and lymphatic endothelial cells - compared to pharyngeal tonsils from the same individual. Conclusions: Despite their physical proximity and histological similarities, palatine and pharyngeal tonsils display marked intra-individual differences in their cellular composition with regard to functionally important immune and stromal subsets. These differences are likely to have immunologic, pathologic, and physiologic significance.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalArchives of Oral Biology
Volume96
DOIs
StatePublished - Dec 1 2018

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Adenoids
Palatine Tonsil
B-Lymphocytes
Immunoglobulin D
Stromal Cells
Individuality
Dendritic Cells
Matched-Pair Analysis
Germinal Center
Flow Cytometry
Endothelial Cells
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Stanisce, Luke ; Sims, Etty ; Hou, Cheryl ; Koshkareva, Yekaterina ; Gaughan, John P. ; Kuzin, Igor ; Bottaro, Andrea. / Differential cellular composition of human palatine and pharyngeal tonsils. In: Archives of Oral Biology. 2018 ; Vol. 96. pp. 80-86.
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Differential cellular composition of human palatine and pharyngeal tonsils. / Stanisce, Luke; Sims, Etty; Hou, Cheryl; Koshkareva, Yekaterina; Gaughan, John P.; Kuzin, Igor; Bottaro, Andrea.

In: Archives of Oral Biology, Vol. 96, 01.12.2018, p. 80-86.

Research output: Contribution to journalArticle

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AU - Stanisce, Luke

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AU - Kuzin, Igor

AU - Bottaro, Andrea

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