Different types of pausing modes during transcription initiation

Eitan Lerner, Antonino Ingargiola, Jookyung J. Lee, Sergei Borukhov, Xavier Michalet, Shimon Weiss

    Research output: Contribution to journalReview articlepeer-review

    9 Scopus citations

    Abstract

    In many cases, initiation is rate limiting to transcription. This due in part to the multiple cycles of abortive transcription that delay promoter escape and the transition from initiation to elongation. Pausing of transcription in initiation can further delay promoter escape. The previously hypothesized pausing in initiation was confirmed by two recent studies from Duchi et al.1 and from Lerner, Chung et al.2 In both studies, pausing is attributed to a lack of forward translocation of the nascent transcript during initiation. However, the two works report on different pausing mechanisms. Duchi et al. report on pausing that occurs during initiation predominantly on-pathway of transcript synthesis. Lerner, Chung et al. report on pausing during initiation as a result of RNAP backtracking, which is off-pathway to transcript synthesis. Here, we discuss these studies, together with additional experimental results from single-molecule FRET focusing on a specific distance within the transcription bubble. We show that the results of these studies are complementary to each other and are consistent with a model involving two types of pauses in initiation: a short-lived pause that occurs in the translocation of a 6-mer nascent transcript and a long-lived pause that occurs as a result of 1–2 nucleotide backtracking of a 7-mer transcript.

    Original languageEnglish (US)
    Pages (from-to)242-253
    Number of pages12
    JournalTranscription
    Volume8
    Issue number4
    DOIs
    StatePublished - Aug 8 2017

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Biochemistry
    • Genetics

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