TY - JOUR
T1 - Development of new inbred transgenic strains of rats with LacZ or GFP
AU - Inoue, Hirokazu
AU - Ohsawa, Ichiro
AU - Murakami, Takashi
AU - Kimura, Atsushi
AU - Hakamata, Yoji
AU - Sato, Yuki
AU - Kaneko, Takashi
AU - Takahashi, Masafumi
AU - Okada, Takashi
AU - Ozawa, Keiya
AU - Francis, Jeremy
AU - Leone, Paola
AU - Kobayashi, Eiji
N1 - Funding Information:
We thank Dr. Masatsugu Udeda (YS New Technology Institute, Tochigi, Japan) for kindly providing GFP transgenic Lewis rats, Dr. Jun-ichi Miyazaki (Osaka University, Graduate School of Medicine, Osaka, Japan) for providing the pCAGGS plasmid, and Dr. Kuniko Shimazaki (Department of Physiology, Jichi Medical School, Tochigi, Japan) for kindly supporting the cerebral stereotactic injection study. We thank Ms. Yasuko Sakuma, Ms. Megumi Hata, and Ms. Harumi Kawana for their skillful technical assistance. This study was supported by grants to E.K. from the Drug Innovation Program of the Japan Health Science Foundation, MEXT, HITEKU (2003), and COE Program (2003). The transgenic rat embryo is available from the Health Science Research Resources Bank at hsrrb@osa.jhsf.or.jp .
PY - 2005/4/1
Y1 - 2005/4/1
N2 - The ideal goal of regeneration medicine is to restore form and function to damaged tissues. While stem cell transplantation is considered a promising therapeutic approach, knowing the fate of transplanted cells using appropriate markers is essential. We developed new inbred transgenic rat strains with lacZ and GFP based on the transgenic (Tg) animal technique in rats. These Tg animals expressed most of their marker genes ubiquitously, compared to previous Tg rats. Immunological antigenicity against marker proteins was evaluated using conventional skin grafting, and results suggested lacZ-Tg-derived skin was much less immunogenic than that of GFP-Tg. However, GFP-positive cells from parental transgenic rats were still potential candidates for the study of cellular fate in immune privilege sites, such as the brain. Taking advantage of less immunogenic lacZ, we also examined the role of bone marrow-derived cells (BMDCs) in skin wound healing using an in vivo biological imaging system. Although transplantation of BMDCs enhanced wound healing at the injection site, BMDCs were detected only for a short time, suggesting a transient contribution of autologous BMDC-transplantation in wound healing. Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation.
AB - The ideal goal of regeneration medicine is to restore form and function to damaged tissues. While stem cell transplantation is considered a promising therapeutic approach, knowing the fate of transplanted cells using appropriate markers is essential. We developed new inbred transgenic rat strains with lacZ and GFP based on the transgenic (Tg) animal technique in rats. These Tg animals expressed most of their marker genes ubiquitously, compared to previous Tg rats. Immunological antigenicity against marker proteins was evaluated using conventional skin grafting, and results suggested lacZ-Tg-derived skin was much less immunogenic than that of GFP-Tg. However, GFP-positive cells from parental transgenic rats were still potential candidates for the study of cellular fate in immune privilege sites, such as the brain. Taking advantage of less immunogenic lacZ, we also examined the role of bone marrow-derived cells (BMDCs) in skin wound healing using an in vivo biological imaging system. Although transplantation of BMDCs enhanced wound healing at the injection site, BMDCs were detected only for a short time, suggesting a transient contribution of autologous BMDC-transplantation in wound healing. Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation.
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U2 - 10.1016/j.bbrc.2005.01.132
DO - 10.1016/j.bbrc.2005.01.132
M3 - Article
C2 - 15721305
AN - SCOPUS:13844256860
SN - 0006-291X
VL - 329
SP - 288
EP - 295
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -