Detection of Several Novel γ‐Aminobutyric Acid‐Containing Compounds in Human CSF

Joanne M. Miller; Thomas N. Ferraro; Theodore A. Hare

doi:10.1111/j.1471-4159.1990.tb04558.x

Detection of Several Novel γ‐Aminobutyric Acid‐Containing Compounds in Human CSF

Joanne M. Miller, Thomas N. Ferraro, Theodore A. Hare

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Abstract: γ‐Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion‐exchange or reverse‐phase chromatography coupled with on‐line alkaline hydrolysis, we have shown 2‐pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA‐containing compounds.

Original language	English (US)
Pages (from-to)	769-773
Number of pages	5
Journal	Journal of Neurochemistry
Volume	55
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-4159.1990.tb04558.x
State	Published - Sep 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1990.tb04558.x

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abstract = "Abstract: γ‐Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion‐exchange or reverse‐phase chromatography coupled with on‐line alkaline hydrolysis, we have shown 2‐pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA‐containing compounds.",

author = "Miller, {Joanne M.} and Ferraro, {Thomas N.} and Hare, {Theodore A.}",

year = "1990",

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doi = "10.1111/j.1471-4159.1990.tb04558.x",

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AU - Miller, Joanne M.

AU - Ferraro, Thomas N.

AU - Hare, Theodore A.

PY - 1990/9

Y1 - 1990/9

N2 - Abstract: γ‐Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion‐exchange or reverse‐phase chromatography coupled with on‐line alkaline hydrolysis, we have shown 2‐pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA‐containing compounds.

AB - Abstract: γ‐Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion‐exchange or reverse‐phase chromatography coupled with on‐line alkaline hydrolysis, we have shown 2‐pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA‐containing compounds.

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Detection of Several Novel γ‐Aminobutyric Acid‐Containing Compounds in Human CSF

Abstract

All Science Journal Classification (ASJC) codes

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