Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes

Cathy F. Yang, Tuzhi Pen, John Schmalzel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A gene sensor was designed using actinomycin D-functionalized magnetic nano-microspheres, which can interact with double-stranded DNAs (dsDNAs) anchored on the gold film electrode of an electrochemical quartz crystal microbalance (EQCM). Actinomycin D acts as a guide that leads heavy microspheres onto the dsDNAs at the EQCM film. A magnetic separation shelf could separate unreacted microspheres conveniently. The modification and DNA hybridization at EQCM electrodes were examined by microgravimetric and electrochemical methods so that an outstanding change in frequency decrease has been detected owing to the mass increase on the EQCM electrodes. The limit for the determination of target DNA could be improved from 6.2 × 10 -8 to 2.0 × 10 -12 mol l -1 by the amplifying technique.

Original languageEnglish (US)
Title of host publicationProceedings of the ISA/IEEE Sensors for Industry Conference
Subtitle of host publicationThe Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04
Pages202-206
Number of pages5
StatePublished - 2004
EventProceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04 - New Orleans, LA, United States
Duration: Jan 27 2004Jan 29 2004

Publication series

NameProceedings of the ISA/IEEE Sensors for Industry Conference

Other

OtherProceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04
Country/TerritoryUnited States
CityNew Orleans, LA
Period1/27/041/29/04

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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