Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes

Cathy F. Yang; Tuzhi Pen; John Schmalzel

Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes

Cathy F. Yang, Tuzhi Pen, John Schmalzel

Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A gene sensor was designed using actinomycin D-functionalized magnetic nano-microspheres, which can interact with double-stranded DNAs (dsDNAs) anchored on the gold film electrode of an electrochemical quartz crystal microbalance (EQCM). Actinomycin D acts as a guide that leads heavy microspheres onto the dsDNAs at the EQCM film. A magnetic separation shelf could separate unreacted microspheres conveniently. The modification and DNA hybridization at EQCM electrodes were examined by microgravimetric and electrochemical methods so that an outstanding change in frequency decrease has been detected owing to the mass increase on the EQCM electrodes. The limit for the determination of target DNA could be improved from 6.2 × 10 ^-8 to 2.0 × 10 ^-12 mol l ^-1 by the amplifying technique.

Original language	English (US)
Title of host publication	Proceedings of the ISA/IEEE Sensors for Industry Conference
Subtitle of host publication	The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04
Pages	202-206
Number of pages	5
State	Published - 2004
Event	Proceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04 - New Orleans, LA, United States Duration: Jan 27 2004 → Jan 29 2004

Publication series

Name	Proceedings of the ISA/IEEE Sensors for Industry Conference

Other

Other	Proceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04
Country/Territory	United States
City	New Orleans, LA
Period	1/27/04 → 1/29/04

All Science Journal Classification (ASJC) codes

General Engineering

Cite this

Yang, C. F., Pen, T., & Schmalzel, J. (2004). Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes. In Proceedings of the ISA/IEEE Sensors for Industry Conference: The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04 (pp. 202-206). (Proceedings of the ISA/IEEE Sensors for Industry Conference).

Yang, Cathy F. ; Pen, Tuzhi ; Schmalzel, John. / Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes. Proceedings of the ISA/IEEE Sensors for Industry Conference: The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04. 2004. pp. 202-206 (Proceedings of the ISA/IEEE Sensors for Industry Conference).

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title = "Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes",

abstract = "A gene sensor was designed using actinomycin D-functionalized magnetic nano-microspheres, which can interact with double-stranded DNAs (dsDNAs) anchored on the gold film electrode of an electrochemical quartz crystal microbalance (EQCM). Actinomycin D acts as a guide that leads heavy microspheres onto the dsDNAs at the EQCM film. A magnetic separation shelf could separate unreacted microspheres conveniently. The modification and DNA hybridization at EQCM electrodes were examined by microgravimetric and electrochemical methods so that an outstanding change in frequency decrease has been detected owing to the mass increase on the EQCM electrodes. The limit for the determination of target DNA could be improved from 6.2 × 10 -8 to 2.0 × 10 -12 mol l -1 by the amplifying technique.",

author = "Yang, {Cathy F.} and Tuzhi Pen and John Schmalzel",

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series = "Proceedings of the ISA/IEEE Sensors for Industry Conference",

pages = "202--206",

booktitle = "Proceedings of the ISA/IEEE Sensors for Industry Conference",

note = "Proceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04 ; Conference date: 27-01-2004 Through 29-01-2004",

}

Yang, CF, Pen, T & Schmalzel, J 2004, Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes. in Proceedings of the ISA/IEEE Sensors for Industry Conference: The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04. Proceedings of the ISA/IEEE Sensors for Industry Conference, pp. 202-206, Proceedings of the ISA/IEEE Sensors for Industry Conference: the Development and usa of Sensors and Transducers, form Theory to the Application of New Technology, SIcon/04, New Orleans, LA, United States, 1/27/04.

Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes. / Yang, Cathy F.; Pen, Tuzhi; Schmalzel, John.
Proceedings of the ISA/IEEE Sensors for Industry Conference: The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04. 2004. p. 202-206 (Proceedings of the ISA/IEEE Sensors for Industry Conference).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - A gene sensor was designed using actinomycin D-functionalized magnetic nano-microspheres, which can interact with double-stranded DNAs (dsDNAs) anchored on the gold film electrode of an electrochemical quartz crystal microbalance (EQCM). Actinomycin D acts as a guide that leads heavy microspheres onto the dsDNAs at the EQCM film. A magnetic separation shelf could separate unreacted microspheres conveniently. The modification and DNA hybridization at EQCM electrodes were examined by microgravimetric and electrochemical methods so that an outstanding change in frequency decrease has been detected owing to the mass increase on the EQCM electrodes. The limit for the determination of target DNA could be improved from 6.2 × 10 -8 to 2.0 × 10 -12 mol l -1 by the amplifying technique.

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Yang CF, Pen T, Schmalzel J. Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes. In Proceedings of the ISA/IEEE Sensors for Industry Conference: The Development and Use of Sensors and Transducers, from Theory to the Application of New Technology, SIcon/04. 2004. p. 202-206. (Proceedings of the ISA/IEEE Sensors for Industry Conference).

Design of a Piezoelectric Gene-sensor Using Base-specific Drug-functionalized Nano-microspheres as Amplifying Probes

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