Derivative matrix isopotential synchronous fluorescence spectroscopy for the direct determination of 1-hydroxypyrene as a urinary biomarker of exposure to polycyclic aromatic hydrocarbons

Y. Q. Li, W. Sui, C. Wu, L. J. Yu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Urinary 1-hydroxypyrene is a biomarker in the measurement of human exposure to polycyclic aromatic hydrocarbons. A rapid and simple derivative isopotential synchronous fluorescence method was developed for the direct determination of 1-hydroxypyrene in urine. A length of iso-intensity route was scanned on the three-dimensional fluorescence spectrum of urine and this result was combined with that from derivative technique. Thus the strong background signals of urine were removed and the 1-hydroxypyrene can be determined directly in urine without tedious pre-separation. The derivative isopotential synchronous fluorescence spectrum was directly obtained from a single scan on a spectrofluorometer, which further simplified isopotential synchronous fluorescence technique. The recoveries of 93% to 115% were obtained for 1-hydroxypyrene added to urine.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalAnalytical Sciences
Volume17
Issue number1
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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