Abstract
In this paper we report the first 1H NMR study of the base-paired secondary structure of yeast 5.8S RNA. On the basis of a combination of homonuclear Overhauser enhancements and temperature dependence of the proton 500-MHz NMR spectrum, we are able to identify and assign eight of the nine base pairs in the most thermally stable helical arm: G116*C137-C117*G136-C118*G135-Cll9*G134-C120-G133-U121*G132-U122*A131-G123*C130. This arm contains an unusually temperature-stable (to 71 °C) segment of four consecutive G*C base pairs. This work constitutes the most direct evidence to date for the existence and base-pair sequence of the GC-rich helix, which is common to most currently popular secondary structural models for eukaryotic 5.8S ribosomal RNA.
Original language | English (US) |
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Pages (from-to) | 8245-8252 |
Number of pages | 8 |
Journal | Biochemistry |
Volume | 25 |
Issue number | 25 |
DOIs | |
State | Published - Dec 1 1986 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biochemistry