Demonstration of the GC-Rich Common Arm in Yeast Ribosomal 5.8S RNA via 500-MHz Proton Nuclear Magnetic Resonance and Overhauser Enhancements

Kai Mon Lee, Alan G. Marshall

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In this paper we report the first 1H NMR study of the base-paired secondary structure of yeast 5.8S RNA. On the basis of a combination of homonuclear Overhauser enhancements and temperature dependence of the proton 500-MHz NMR spectrum, we are able to identify and assign eight of the nine base pairs in the most thermally stable helical arm: G116*C137-C117*G136-C118*G135-Cll9*G134-C120-G133-U121*G132-U122*A131-G123*C130. This arm contains an unusually temperature-stable (to 71 °C) segment of four consecutive G*C base pairs. This work constitutes the most direct evidence to date for the existence and base-pair sequence of the GC-rich helix, which is common to most currently popular secondary structural models for eukaryotic 5.8S ribosomal RNA.

Original languageEnglish (US)
Pages (from-to)8245-8252
Number of pages8
JournalBiochemistry
Volume25
Issue number25
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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