Deconvolution of mixed isotope distribution patterns for quantitative proteomics

Steven H. Seeholzer; Anthony T. Yeung; Randy Strich

Deconvolution of mixed isotope distribution patterns for quantitative proteomics

Steven H. Seeholzer, Anthony T. Yeung, Randy Strich

Research output: Contribution to conference › Paper › peer-review

Abstract

The proteins and their modified forms were analyzed and a change in a cell's phenotype was correlated with changes in protein expression patterns in proteonomics research. 2D electrophoresis and mass spectrometry were used for the study. The fractional ¹⁵N-enriched proteins extracted from separate cultures were mixed in various ratios and resolved by 2D electrophoresis with cysteine reduction and alkylation. It was observed that the main advantage of this approach was that the monoisotopic mass information was preserved for all protein expression ratios and allowed facile identification of the protein from peptide mass mapping.

Original language	English (US)
Pages	329-330
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

All Science Journal Classification (ASJC) codes

Spectroscopy

Cite this

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title = "Deconvolution of mixed isotope distribution patterns for quantitative proteomics",

abstract = "The proteins and their modified forms were analyzed and a change in a cell's phenotype was correlated with changes in protein expression patterns in proteonomics research. 2D electrophoresis and mass spectrometry were used for the study. The fractional 15N-enriched proteins extracted from separate cultures were mixed in various ratios and resolved by 2D electrophoresis with cysteine reduction and alkylation. It was observed that the main advantage of this approach was that the monoisotopic mass information was preserved for all protein expression ratios and allowed facile identification of the protein from peptide mass mapping.",

author = "Seeholzer, {Steven H.} and Yeung, {Anthony T.} and Randy Strich",

year = "2002",

language = "English (US)",

pages = "329--330",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Deconvolution of mixed isotope distribution patterns for quantitative proteomics

AU - Seeholzer, Steven H.

AU - Yeung, Anthony T.

AU - Strich, Randy

PY - 2002

Y1 - 2002

N2 - The proteins and their modified forms were analyzed and a change in a cell's phenotype was correlated with changes in protein expression patterns in proteonomics research. 2D electrophoresis and mass spectrometry were used for the study. The fractional 15N-enriched proteins extracted from separate cultures were mixed in various ratios and resolved by 2D electrophoresis with cysteine reduction and alkylation. It was observed that the main advantage of this approach was that the monoisotopic mass information was preserved for all protein expression ratios and allowed facile identification of the protein from peptide mass mapping.

AB - The proteins and their modified forms were analyzed and a change in a cell's phenotype was correlated with changes in protein expression patterns in proteonomics research. 2D electrophoresis and mass spectrometry were used for the study. The fractional 15N-enriched proteins extracted from separate cultures were mixed in various ratios and resolved by 2D electrophoresis with cysteine reduction and alkylation. It was observed that the main advantage of this approach was that the monoisotopic mass information was preserved for all protein expression ratios and allowed facile identification of the protein from peptide mass mapping.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442666863&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442666863

SP - 329

EP - 330

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Deconvolution of mixed isotope distribution patterns for quantitative proteomics

Abstract

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All Science Journal Classification (ASJC) codes

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