Deconvolution of mixed isotope distribution patterns for quantitative proteomics

Steven H. Seeholzer, Anthony T. Yeung, Randy Strich

Research output: Contribution to conferencePaperpeer-review

Abstract

The proteins and their modified forms were analyzed and a change in a cell's phenotype was correlated with changes in protein expression patterns in proteonomics research. 2D electrophoresis and mass spectrometry were used for the study. The fractional 15N-enriched proteins extracted from separate cultures were mixed in various ratios and resolved by 2D electrophoresis with cysteine reduction and alkylation. It was observed that the main advantage of this approach was that the monoisotopic mass information was preserved for all protein expression ratios and allowed facile identification of the protein from peptide mass mapping.

Original languageEnglish (US)
Pages329-330
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period6/2/026/6/02

All Science Journal Classification (ASJC) codes

  • Spectroscopy

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