Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements

Jeffrey Viviano; Hao Wu; Anuradha Krishnan; Kandalam Ramanujachary; Venkat Venkataraman

doi:10.1016/j.dib.2016.01.030

Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements

Jeffrey Viviano, Hao Wu, Anuradha Krishnan, Kandalam Ramanujachary, Venkat Venkataraman

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Abstract

In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins. NCS proteins exhibit calcium-dependent mobility shift in native gels. The sensitivity of this shift to calcium necessitated a precise determination of calcium concentrations in all reagents used. We determined the calcium concentrations in different components used along with the samples in the native gel experiments. These were: 20 mM Tris pH 7.5, loading dye and running buffer, with distilled water as reference. Calcium determinations were through ICP measurements. It was found that the running buffer contained calcium (244 nM) over the blank.

Original language	English (US)
Pages (from-to)	820-822
Number of pages	3
Journal	Data in Brief
Volume	6
DOIs	https://doi.org/10.1016/j.dib.2016.01.030
State	Published - Mar 1 2016

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Viviano, J., Wu, H., Krishnan, A., Ramanujachary, K., & Venkataraman, V. (2016). Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements. Data in Brief, 6, 820-822. https://doi.org/10.1016/j.dib.2016.01.030

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AU - Wu, Hao

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AU - Ramanujachary, Kandalam

AU - Venkataraman, Venkat

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