GreA is a 17.6 kDa protein from Escherichia coli that induces cleavage of the nascent transcript in the elongating complex of RNA polymerase, followed by release of the 3'-terminal fragment. Crystals of GreA have been obtained from polyethylene glycol 4000, 2-propanol and sodium citrate, pH 5.6 and have been propogated by a novel seeding procedure. The crystals diffract beyond 2 AÅ resolution and belong to the orthorhombic space group P212121, with cell dimensions a = 101.7 AÅ, b = 42.22 AÅ, c = 40.05 AÅ and with one molecule in the asymmetric unit.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology