Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex

Dmitry Temiakov, Tahir H. Tahirov, Michael Anikin, William T. McAllister, Dmitry G. Vassylyev, Shigeyuki Yokoyama

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A phage T7 RNA polymerase elongation complex containing 8 bp of RNA-DNA hybrid and 10 bp of downstream DNA with a total molecular mass of ∼110 kDa was crystallized. A complete diffraction data set was collected at 2.6 Å resolution. The structure determination and refinement are in progress.

Original languageEnglish (US)
Pages (from-to)185-187
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology

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