Crystal structure of the GreA transcript cleavage factor from escherichia coli

Charles E. Stebbins, Sergei Borukhov, Marianna Orlova, Andrey Polyakov, Alex Goldfarb, Seth A. Darst

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

TRANSCRIPTION elongation factors stimulate the activity of DNA-dependent RNA polymerases by increasing the overall elongation rate and the completion of RNA chains. One group of such factors, which includes Escherichia coli GreA, GreB and eukaryotic SII (TFIIS), acts by inducing hydrolytic cleavage of the transcript within the RNA polymerase, followed by relase of the 3–-terminal fragment1–5. Here we report the crystal structure of GreA at 2.2 Å resolution. The structure contains an amino-terminal domain consisting of an antiparallel α-helical coiled-coil dimer which extends into solution, reminiscent of the coiled coil in seryl-tRNA synthetases6. A site near the tip of the coiled-coil “finger” plays a direct role in the transcript cleavage reaction by contacting the 3’-end of the transcript. The structure exhibits an unusual asymmetric charge distribution which indicates the manner in which GreA interacts with the RNA polymerase elongation complex.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalNature
Volume373
Issue number6515
DOIs
StatePublished - Feb 16 1995

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DNA-Directed RNA Polymerases
Escherichia coli
Peptide Elongation Factors
Transfer RNA
Fingers
RNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Stebbins, C. E., Borukhov, S., Orlova, M., Polyakov, A., Goldfarb, A., & Darst, S. A. (1995). Crystal structure of the GreA transcript cleavage factor from escherichia coli. Nature, 373(6515), 636-640. https://doi.org/10.1038/373636a0
Stebbins, Charles E. ; Borukhov, Sergei ; Orlova, Marianna ; Polyakov, Andrey ; Goldfarb, Alex ; Darst, Seth A. / Crystal structure of the GreA transcript cleavage factor from escherichia coli. In: Nature. 1995 ; Vol. 373, No. 6515. pp. 636-640.
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Stebbins, CE, Borukhov, S, Orlova, M, Polyakov, A, Goldfarb, A & Darst, SA 1995, 'Crystal structure of the GreA transcript cleavage factor from escherichia coli', Nature, vol. 373, no. 6515, pp. 636-640. https://doi.org/10.1038/373636a0

Crystal structure of the GreA transcript cleavage factor from escherichia coli. / Stebbins, Charles E.; Borukhov, Sergei; Orlova, Marianna; Polyakov, Andrey; Goldfarb, Alex; Darst, Seth A.

In: Nature, Vol. 373, No. 6515, 16.02.1995, p. 636-640.

Research output: Contribution to journalArticle

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Stebbins CE, Borukhov S, Orlova M, Polyakov A, Goldfarb A, Darst SA. Crystal structure of the GreA transcript cleavage factor from escherichia coli. Nature. 1995 Feb 16;373(6515):636-640. https://doi.org/10.1038/373636a0