Combinatorial library of lipidoids for in vitro dna delivery

Shuo Sun, Ming Wang, Sarah A. Knupp, Yadira Soto-Feliciano, Xiao Hu, David L. Kaplan, Robert Langer, Daniel G. Anderson, Qiaobing Xu

    Research output: Contribution to journalArticlepeer-review

    55 Scopus citations

    Abstract

    A combinatorial library of lipidoids was constructed and studied for in vitro gene delivery. The library of lipidoids was synthesized by reacting commercially available amines with lipophilic acrylates, acrylamides, or epoxides. Lipidoids derived from amine 86 (N,N-bis(2-hydroxyethyl)ethylene diamine) and amine 87 (N-(3-aminopropyl)diethaneamine) showed high efficiency in DNA delivery, some with a higher transfection efficiency than Lipofectamine 2000, a commonly used commercial gold standard for in vitro gene delivery. The structure-activity relationship between the lipidoids was further studied with respect to small variations in chemical structures and the resulting efficiency in DNA delivery in vitro. Since these lipidoids are easy to synthesize and do not require a colipid for efficient DNA delivery, they could offer an inexpensive but effective alternative to other commonly used commercial gene delivery carriers.

    Original languageEnglish (US)
    Pages (from-to)135-140
    Number of pages6
    JournalBioconjugate Chemistry
    Volume23
    Issue number1
    DOIs
    StatePublished - Jan 18 2012

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Pharmacology
    • Pharmaceutical Science
    • Organic Chemistry

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