Chromatographic behaviour of human leukocyte α-interferon A on adsorbents with sepharose and silochrome matrices

S. I. Borukhov, L. S. Izotova, S. V. Kostrov

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Abstract

Chromatography on immobilized monoclonal antibodies NK-2 from a bacterial strain-producer resulted in a pure human leukocyte α-interferon A (α-INF-A) homogeneous upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and reverse phase high performance liquid chromatography. The chromatographic properties of partially purified α-INF-A on synthetic and commercial sorbents containing immobilized dyes, aromatic dipeptides, chelating and hydrophobic ligands as well as on porous glass have been investigated. In most cases, [125I]α-INF-A was used as an inner standard. The chromatographic behaviour of native and [125I]-labeled α-INF-A was practically the same, α-INF-A was most effectively chromatographed on porous glass, L-Trp-L-Trp-Sepharose 4B and Cu2+-chelate sorbents. In the latter case, the feasibility of substitution of the Sepharose matrix for the silochrome one has been demonstrated. It has been proposed that α-INF-A has a hydrophobic 'pocket' with exposed aromatic amino acid residues which are capable of selective binding to aromatic dipeptides.

Original languageEnglish (US)
Pages (from-to)1471-1480
Number of pages10
JournalBiokhimiya
Volume50
Issue number9
StatePublished - Jan 1 1985

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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