Chemical modification of the recombinant human αA- and β-interferons

Sergey I. Borukhov, Alexander Ya Strongin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chemical modification has been used to map the residues essential for the antiviral activity of the recombinant human αA- and β-interferons. Modification of His residues with diethylpyrocarbonate and Nα-tosyl-L-lysyl chloromethylketone does not inhibit both interferons, whereas Nα-tosyl-L-phenylalanyl chloromethylketone significantly suppressing the activity of β-interferon does not affect the activity of αA-interferon. After the modification of 1, 2 and 3 Lys residues from 11 ones with 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester αA-interferon reveals 100%, 50% and 10% of the initial activity, respectively. Modification of Trp residues with H2O2, 2-nitrobenzenesulfenyl chloride or 2-hydroxy-5-nitrobenzylbromide inactivates αA- and β-interferons completely. Presumably Trp residue(s) is essential for the antiviral activity of α- and β-interferons.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number1
DOIs
StatePublished - Feb 28 1990

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Chemical modification
Interferons
Antiviral Agents
Diethyl Pyrocarbonate
Human Activities
Esters

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Chemical modification of the recombinant human αA- and β-interferons",
abstract = "Chemical modification has been used to map the residues essential for the antiviral activity of the recombinant human αA- and β-interferons. Modification of His residues with diethylpyrocarbonate and Nα-tosyl-L-lysyl chloromethylketone does not inhibit both interferons, whereas Nα-tosyl-L-phenylalanyl chloromethylketone significantly suppressing the activity of β-interferon does not affect the activity of αA-interferon. After the modification of 1, 2 and 3 Lys residues from 11 ones with 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester αA-interferon reveals 100{\%}, 50{\%} and 10{\%} of the initial activity, respectively. Modification of Trp residues with H2O2, 2-nitrobenzenesulfenyl chloride or 2-hydroxy-5-nitrobenzylbromide inactivates αA- and β-interferons completely. Presumably Trp residue(s) is essential for the antiviral activity of α- and β-interferons.",
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Chemical modification of the recombinant human αA- and β-interferons. / Borukhov, Sergey I.; Strongin, Alexander Ya.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 1, 28.02.1990, p. 74-80.

Research output: Contribution to journalArticle

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AU - Strongin, Alexander Ya

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