Characterization of injectable hydrogels based on poly(N- isopropylacrylamide)-g-chondroitin sulfate with adhesive properties for nucleus pulposus tissue engineering

Craig Wiltsey, Pamela Kubinski, Thomas Christiani, Katelynn Toomer, Joseph Sheehan, Amanda Branda, Jennifer Kadlowec, Cristina Iftode, Andrea Vernengo

Research output: Contribution to journalArticle

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Abstract

The goal of this work is to develop an injectable nucleus pulposus (NP) tissue engineering scaffold with the ability to form an adhesive interface with surrounding disc tissue. A family of in situ forming hydrogels based on poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAm-g-CS) were evaluated for their mechanical properties, bioadhesive strength, and cytocompatibility. It was shown experimentally and computationally with the Neo-hookean hyperelastic model that increasing the crosslink density and decreasing the CS concentration increased mechanical properties at 37 °C, generating several hydrogel formulations with unconfined compressive modulus values similar to what has been reported for the native NP. The adhesive tensile strength of PNIPAAm increased significantly with CS incorporation (p < 0.05), ranging from 0.4 to 1 kPa. Live/Dead and XTT assay results indicate that the copolymer is not cytotoxic to human embryonic kidney (HEK) 293 cells. Taken together, these data indicate the potential of PNIPAAm-g-CS to function as a scaffold for NP regeneration.

Original languageEnglish (US)
Pages (from-to)837-847
Number of pages11
JournalJournal of Materials Science: Materials in Medicine
Volume24
Issue number4
DOIs
StatePublished - Apr 1 2013

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Biomedical Engineering

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