Changes in water structure induced by the guanidinium cation and implications for protein denaturation

J. Nathan Scott, Nathaniel V. Nucci, Jane M. Vanderkooi

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The effect of the guanidinium cation on the hydrogen bonding strength of water was analyzed using temperature-excursion Fourier transform infrared spectra of the OH stretching vibration in 5% H2O/95% D2O solutions containing a range of different guanidine-HCl and guanidine-HBr concentrations. Our findings indicate that the guanidinium cation causes the water H-bonds in solution to become more linear than those found in bulk water, and that it also inhibits the response of the H-bond network to increased temperature. Quantum chemical calculations also reveal that guanidinium affects both the charge distribution on water molecules directly H-bonded to it as well as the OH stretch frequency of H-bonds in which that water molecule is the donor. The implications of our findings to hydrophobic solvation and protein denaturation are discussed.

Original languageEnglish (US)
Pages (from-to)10939-10948
Number of pages10
JournalJournal of Physical Chemistry A
Volume112
Issue number43
DOIs
StatePublished - Oct 30 2008

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry

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