Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection

Claude Krummenacher, Isabelle Baribaud, Roselyn J. Eisenberg, Gary H. Cohen

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

During viral entry, herpes simplex virus (HSV) glycoprotein D (gD) interacts with a specific cellular receptor such as nectin-1 (PRR1/HveC/CD111) or the herpesvirus entry mediator A (HVEM/HveA). Nectin-1 is involved in cell-to-cell adhesion. It is located at adherens junctions, where it bridges cells through homophilic or heterophilic interactions with other nectins. Binding of HSV gD prevents nectin-1-mediated cell aggregation. Since HSV gD affects the natural function of nectin-1, we further investigated the effects of gD expression on nectin-1 during HSV infection or in transfected cells. We also studied the importance of the interaction between nectin-1 and the cytoplasmic protein afadin for HSV entry and spread as well as the effects of infection on this interaction. In these investigations, we used a panel of cells expressing nectin-1 or nectin-1-green fluorescent protein fusions as the only mediators of HSV entry. During HSV infection, nectin-1 localization at adherens junction was dramatically altered in a manner dependent on gD expression. Nectin-1 and gD colocalized at cell contact areas between infected and noninfected cells and at the edges of plaques. This specific accumulation of gD at junctions was driven by expression of nectin-1 in trans on the surface of adjacent cells. Reciprocally, nectin-1 was maintained at junctions by the trans expression of gD in the absence of a cellular natural ligand. Our observations indicate that newly synthesized gD substitutes for nectin-1 of infected cells at junctions with noninfected cells. We propose that gD attracts and maintains the receptor at junctions where it can be used for virus spread.

Original languageEnglish (US)
Pages (from-to)8985-8999
Number of pages15
JournalJournal of Virology
Volume77
Issue number16
DOIs
StatePublished - Aug 1 2003

Fingerprint

herpes simplex
Virus Diseases
Simplexvirus
glycoproteins
Glycoproteins
viruses
infection
cells
Adherens Junctions
Virus Internalization
nectins
Human herpesvirus 1
Receptors, Tumor Necrosis Factor, Member 14
receptors
green fluorescent protein
cell adhesion
Cell Aggregation
Intercellular Junctions
Green Fluorescent Proteins
Cell Adhesion

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Krummenacher, Claude ; Baribaud, Isabelle ; Eisenberg, Roselyn J. ; Cohen, Gary H. / Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection. In: Journal of Virology. 2003 ; Vol. 77, No. 16. pp. 8985-8999.
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Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection. / Krummenacher, Claude; Baribaud, Isabelle; Eisenberg, Roselyn J.; Cohen, Gary H.

In: Journal of Virology, Vol. 77, No. 16, 01.08.2003, p. 8985-8999.

Research output: Contribution to journalArticle

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