Cardiovascular and inflammatory changes do not correlate with tissue NO levels in acute endotoxemia

K. Yin, C. E. Hock, M. Tahamont, P. Y.K. Wong

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) has been implicated in the development of circulatory dysfunction as well as being a toxic free radical species in endotoxemia. We studied the time course of cardiovascular changes and correlated this to tissue nitrite production (index of nitric oxide) and myeloperoxidase (MPO; index of neutrophil infiltration) in acute endotoxemia. Rats were instrumented for measurement of MABP and CO before administration of lipopolysaccharide (LPS) bolus (salmonella enteritidis. 10 mg/kg, i.v.). Control rats received saline. Rats were sacrificed at 1h, 2h and 3h after LPS administration. At 1h, MABP and CO were decreased by 53 and 61% (P<0.01) respectively, compared to controls. Tissue nitrite in lung, heart and intestine were not altered. MPO was slightly increased in intestine of LPS rats. At 2h, MABP and CO were decreased by 27 and 47% respectively. Lung nitrite of LPS treated rats was increased 2-fold but there were no changes in MPO while circulating neutrophils decreased by 67%. Nitrite levels were elevated in all tissues from LPS treated rats at 3h while MPO was increased only in the lung. Circulating neutrophils were decreased by 72%. MABP and CO were depressed to the same degree as at 2h. These results suggest that cardiovascular and inflammatory changes do not correlate with increases in tissue NO levels in acute endotoxemia.

Original languageEnglish (US)
Pages (from-to)A598
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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