Bovine leukemia virus long terminal repeat: A cell type-specific promoter

David Derse, Salvatore J. Caradonna, James W. Casey

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The functional activity of the promoter unit contained within the long terminal repeat (LTR) of bovine leukemia virus (BLV) was examined by monitoring transient expression of a heterologous gene placed under its control. Various cell lines were transfected with recombinant plasmids carrying the bacterial chloramphenicol acetyltransferase (CAT) gene coupled to the BLV LTR (pBL-cat). Transient expression of CAT activity directed by the BLV LTR was observed only in the established BLV-producer cell lines derived from fetal lamb kidney (FLK) cells and bat lung cells. The amount of CAT activity transiently expressed in FLK-BLV cells was decreased approximately tenfold by deletion ofLTR sequences located within a region 100 to 170 nucleotides upstream of the RNA start site. Surprisingly, removal of the region 50 base pairs downstream ofthe RNA initiation site to the 3′- end of the LTR reduced the expression of CAT activity by 87 percent. The BLV LTR thus appears to be an unusual promoter unit, functioning in a cell type-specific manner and possessing sequences on both the 5′ and 3′ sides of the RNA start site that influence gene expression.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalScience
Volume227
Issue number4684
DOIs
StatePublished - 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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