TY - GEN
T1 - Biomimetic fabrication and freeze-casting of collagen-apatite hydrogels for bone tissue engineering
AU - Xia, Zengmin
AU - Wei, Mei
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/12/2
Y1 - 2014/12/2
N2 - Collagen-apatite (Col-Ap) scaffolds have been widely employed for bone tissue engineering. In the current study, we fabricated Col-Ap hydrogels with a biomimetic structure and freeze-dried Col-Ap hydrogels with a hierarchical lamellar structure. Col-Ap hydrogel was prepared using a biomimetic approach combining self-assembling of collagen molecules with simultaneous mineralization in a modified simulated body fluid (m-SBF). A novel two-temperature process involving gelation at 25'C and then 40'C was applied to increase the diameter of mineralized collagen fibers. Col-Ap hydrogel consisting of collagen bundles reinforced by apatite nano-particles was further subjected to self-compression and controlled freeze-casting. The freeze-dried hydrogel exhibits a hierarchical lamellar structure consisting of co-aligned macro and micro pores. Moreover, this novel lamellar structured scaffold supports the attachment and spreading of MC3T3-E1osteoblasts. Therefore, owing to the biomimetic composition, hierarchical lamellar structure and good biocompatibility of this novel freeze-dried Col-Ap hydrogel, it has great potential to be used in bone tissue engineering applications.
AB - Collagen-apatite (Col-Ap) scaffolds have been widely employed for bone tissue engineering. In the current study, we fabricated Col-Ap hydrogels with a biomimetic structure and freeze-dried Col-Ap hydrogels with a hierarchical lamellar structure. Col-Ap hydrogel was prepared using a biomimetic approach combining self-assembling of collagen molecules with simultaneous mineralization in a modified simulated body fluid (m-SBF). A novel two-temperature process involving gelation at 25'C and then 40'C was applied to increase the diameter of mineralized collagen fibers. Col-Ap hydrogel consisting of collagen bundles reinforced by apatite nano-particles was further subjected to self-compression and controlled freeze-casting. The freeze-dried hydrogel exhibits a hierarchical lamellar structure consisting of co-aligned macro and micro pores. Moreover, this novel lamellar structured scaffold supports the attachment and spreading of MC3T3-E1osteoblasts. Therefore, owing to the biomimetic composition, hierarchical lamellar structure and good biocompatibility of this novel freeze-dried Col-Ap hydrogel, it has great potential to be used in bone tissue engineering applications.
UR - http://www.scopus.com/inward/record.url?scp=84940706084&partnerID=8YFLogxK
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U2 - 10.1109/NEBEC.2014.6972981
DO - 10.1109/NEBEC.2014.6972981
M3 - Conference contribution
AN - SCOPUS:84940706084
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
BT - Proceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
Y2 - 25 April 2014 through 27 April 2014
ER -