Binding of the initiation factor σ70 to core RNA polymerase is a multistep process

Tanja M. Gruber; Dmitriy Markov; Meghan M. Sharp; Brian A. Young; Chi Zen Lu; Hong Ji Zhong; Irina Artsimovitch; Katherine M. Geszvain; Terrance M. Arthur; Richard R. Burgess; Robert Landick; Konstantin Severinov; Carol A. Gross

doi:10.1016/S1097-2765(01)00292-1

Binding of the initiation factor σ⁷⁰ to core RNA polymerase is a multistep process

Tanja M. Gruber, Dmitriy Markov, Meghan M. Sharp, Brian A. Young, Chi Zen Lu, Hong Ji Zhong, Irina Artsimovitch, Katherine M. Geszvain, Terrance M. Arthur, Richard R. Burgess, Robert Landick, Konstantin Severinov, Carol A. Gross

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and σ⁷⁰, the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between σ⁷⁰ and specific domains of RNA polymerase and provide evidence that σ⁷⁰ and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of σ⁷⁰ with core RNA polymerase is associated with progression through the process of transcription initiation.

Original language	English (US)
Pages (from-to)	21-31
Number of pages	11
Journal	Molecular Cell
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/S1097-2765(01)00292-1
State	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1016/S1097-2765(01)00292-1

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title = "Binding of the initiation factor σ70 to core RNA polymerase is a multistep process",

abstract = "The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and σ70, the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between σ70 and specific domains of RNA polymerase and provide evidence that σ70 and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of σ70 with core RNA polymerase is associated with progression through the process of transcription initiation.",

author = "Gruber, {Tanja M.} and Dmitriy Markov and Sharp, {Meghan M.} and Young, {Brian A.} and Lu, {Chi Zen} and Zhong, {Hong Ji} and Irina Artsimovitch and Geszvain, {Katherine M.} and Arthur, {Terrance M.} and Burgess, {Richard R.} and Robert Landick and Konstantin Severinov and Gross, {Carol A.}",

year = "2001",

doi = "10.1016/S1097-2765(01)00292-1",

language = "English (US)",

volume = "8",

pages = "21--31",

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TY - JOUR

T1 - Binding of the initiation factor σ70 to core RNA polymerase is a multistep process

AU - Gruber, Tanja M.

AU - Markov, Dmitriy

AU - Sharp, Meghan M.

AU - Young, Brian A.

AU - Lu, Chi Zen

AU - Zhong, Hong Ji

AU - Artsimovitch, Irina

AU - Geszvain, Katherine M.

AU - Arthur, Terrance M.

AU - Burgess, Richard R.

AU - Landick, Robert

AU - Severinov, Konstantin

AU - Gross, Carol A.

PY - 2001

Y1 - 2001

N2 - The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and σ70, the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between σ70 and specific domains of RNA polymerase and provide evidence that σ70 and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of σ70 with core RNA polymerase is associated with progression through the process of transcription initiation.

AB - The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and σ70, the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between σ70 and specific domains of RNA polymerase and provide evidence that σ70 and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of σ70 with core RNA polymerase is associated with progression through the process of transcription initiation.

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DO - 10.1016/S1097-2765(01)00292-1

M3 - Article

C2 - 11511357

AN - SCOPUS:17944375388

SN - 1097-2765

VL - 8

SP - 21

EP - 31

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Binding of the initiation factor σ⁷⁰ to core RNA polymerase is a multistep process

Abstract

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