Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

A. A. Novikov; S. I. Borukhov; A. Ya Strongin

doi:10.1016/0006-291X(90)91467-7

Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

A. A. Novikov, S. I. Borukhov, A. Ya Strongin

Cell Biology

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.

Original language	English (US)
Pages (from-to)	297-301
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91467-7
State	Published - May 31 1990

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All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Novikov, A. A., Borukhov, S. I., & Strongin, A. Y. (1990). Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells. Biochemical and Biophysical Research Communications, 169(1), 297-301. https://doi.org/10.1016/0006-291X(90)91467-7

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10.1016/0006-291X(90)91467-7