Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

A. A. Novikov; S. I. Borukhov; A. Ya Strongin

doi:10.1016/0006-291X(90)91467-7

Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

A. A. Novikov, S. I. Borukhov, A. Ya Strongin

Cell Biology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.

Original language	English (US)
Pages (from-to)	297-301
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91467-7
State	Published - May 31 1990

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(90)91467-7

Fingerprint Dive into the research topics of 'Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells'. Together they form a unique fingerprint.

Cite this

@article{9038ebe4649440678162db0c7fdb7883,

title = "Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells",

abstract = "The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.",

author = "Novikov, {A. A.} and Borukhov, {S. I.} and Strongin, {A. Ya}",

year = "1990",

month = may,

day = "31",

doi = "10.1016/0006-291X(90)91467-7",

language = "English (US)",

volume = "169",

pages = "297--301",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

AU - Novikov, A. A.

AU - Borukhov, S. I.

AU - Strongin, A. Ya

PY - 1990/5/31

Y1 - 1990/5/31

N2 - The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.

AB - The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.

UR - http://www.scopus.com/inward/record.url?scp=0025297529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025297529&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(90)91467-7

DO - 10.1016/0006-291X(90)91467-7

M3 - Article

C2 - 2112381

AN - SCOPUS:0025297529

VL - 169

SP - 297

EP - 301

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Cite this