Abstract
The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 297-301 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 169 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 31 1990 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells. / Novikov, A. A.; Borukhov, S. I.; Strongin, A. Ya.
In: Biochemical and Biophysical Research Communications, Vol. 169, No. 1, 31.05.1990, p. 297-301.Research output: Contribution to journal › Article
TY - JOUR
T1 - Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cells
AU - Novikov, A. A.
AU - Borukhov, S. I.
AU - Strongin, A. Ya
PY - 1990/5/31
Y1 - 1990/5/31
N2 - The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
AB - The plasmid pBINSi, containing the promoter, SD and leader peptide sequences of Bacillusamyloliquefaciens α-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of proteasedeficient Bacillussubtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
UR - http://www.scopus.com/inward/record.url?scp=0025297529&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025297529&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91467-7
DO - 10.1016/0006-291X(90)91467-7
M3 - Article
C2 - 2112381
AN - SCOPUS:0025297529
VL - 169
SP - 297
EP - 301
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -